Activity stain for the detection of cyclic nucleotide phosphodiesterase separated by polyacrylamide gel electrophoresis and its application to the cyclic nucleotide phosphodiesterase of beef heart

Elihu N. Goren, Allen H. Hirsch, Ora M. Rosen

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

A rapid method for detecting cyclic 3′,5′-nucleotide phosphodiesterases in a gel matrix after electrophoresis has been described. The method can be employed for identifying cyclic nucleotide phosphodiesterase activity in partially purified preparations as well as for screening crude tissue extracts for this activity. When combined with density gel scanning, this technique permits the estimation of the relative levels of enzyme activity in different tissues or preparations. Beef heart contains at least two electrophoretically distinct forms of cyclic nucleotide phosphodiesterase activity, both of which can hydrolyze cyclic 3′,5′-AMP and cyclic 3′,5′-GMP.

Original languageEnglish (US)
Pages (from-to)156-161
Number of pages6
JournalAnalytical Biochemistry
Volume43
Issue number1
DOIs
StatePublished - 1971

Fingerprint

Beef
Cyclic Nucleotides
Phosphoric Diester Hydrolases
Electrophoresis
Polyacrylamide Gel Electrophoresis
Coloring Agents
Gels
Type 5 Cyclic Nucleotide Phosphodiesterases
Guanosine Monophosphate
Tissue Extracts
Complex Mixtures
Cyclic AMP
Nucleotides
Enzyme activity
Screening
Enzymes
Tissue
Scanning
Red Meat
polyacrylamide gels

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

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AU - Rosen, Ora M.

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