Activation of NF-kappa B in the mouse retina after ischemia reperfusion

Yue guo Chen, Cheng Zhang, Ting huai Wu, Jian sen Jiang, Mark Tso

Research output: Contribution to journalArticle

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Abstract

OBJECTIVE: To investigate the expression of NF-kappa B following retinal ischemia and reperfusion injury in mice. METHODS: Retinal ischemia was induced by elevation of intraocular pressure. Retinal degeneration and atrophy were quantified by an image analysis system. Immunohistochemistry using p65 monoclonal antibody was performed on the retina and co-related with TUNEL labeling. RESULTS: Inner retinal thickness was increased in the initial 24 hours following retinal ischemia and was ascribed to tissue edema, but was significantly decreased by 168 hours after reperfusion. Six hours after retinal ischemia, p65 immunoreactivity was increased in the ganglion cell and the inner nuclear layers, reached a peak at 24 hours, and was parallel to TUNEL labeling. Double labeling with p65 and TUNEL showed partial co-localization of p65 and TUNEL labeling, predominantly in the inner nuclear layer. CONCLUSIONS: Activation of NF-kappa B appears to play an important role in retinal degeneration following retinal ischemia and reperfusion injury. The pro- and anti-apoptotic effects of NF-kappa B after retinal ischemia are being further investigated.

Original languageEnglish (US)
Pages (from-to)560-564
Number of pages5
Journal[Zhonghua yan ke za zhi] Chinese journal of ophthalmology
Volume39
Issue number9
StatePublished - Sep 2003
Externally publishedYes

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NF-kappa B
In Situ Nick-End Labeling
Reperfusion
Retina
Ischemia
Retinal Degeneration
Reperfusion Injury
Intraocular Pressure
Ganglia
Atrophy
Edema
Immunohistochemistry
Monoclonal Antibodies

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Activation of NF-kappa B in the mouse retina after ischemia reperfusion. / Chen, Yue guo; Zhang, Cheng; Wu, Ting huai; Jiang, Jian sen; Tso, Mark.

In: [Zhonghua yan ke za zhi] Chinese journal of ophthalmology, Vol. 39, No. 9, 09.2003, p. 560-564.

Research output: Contribution to journalArticle

Chen, Yue guo ; Zhang, Cheng ; Wu, Ting huai ; Jiang, Jian sen ; Tso, Mark. / Activation of NF-kappa B in the mouse retina after ischemia reperfusion. In: [Zhonghua yan ke za zhi] Chinese journal of ophthalmology. 2003 ; Vol. 39, No. 9. pp. 560-564.
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AB - OBJECTIVE: To investigate the expression of NF-kappa B following retinal ischemia and reperfusion injury in mice. METHODS: Retinal ischemia was induced by elevation of intraocular pressure. Retinal degeneration and atrophy were quantified by an image analysis system. Immunohistochemistry using p65 monoclonal antibody was performed on the retina and co-related with TUNEL labeling. RESULTS: Inner retinal thickness was increased in the initial 24 hours following retinal ischemia and was ascribed to tissue edema, but was significantly decreased by 168 hours after reperfusion. Six hours after retinal ischemia, p65 immunoreactivity was increased in the ganglion cell and the inner nuclear layers, reached a peak at 24 hours, and was parallel to TUNEL labeling. Double labeling with p65 and TUNEL showed partial co-localization of p65 and TUNEL labeling, predominantly in the inner nuclear layer. CONCLUSIONS: Activation of NF-kappa B appears to play an important role in retinal degeneration following retinal ischemia and reperfusion injury. The pro- and anti-apoptotic effects of NF-kappa B after retinal ischemia are being further investigated.

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