Activation of LKB1-Akt pathway independent of phosphoinositide 3-kinase plays a critical role in the proliferation of hepatocellular carcinoma from nonalcoholic steatohepatitis

Nuria MartíNez-LóPez, Marta Varela-Rey, David FernáNdez-Ramos, Ashwin Woodhoo, Mercedes VáZquez-Chantada, Nieves Embade, Luis Espinosa-Hevia, Francisco Javier Bustamante, Luis A. Parada, Manuel S. Rodriguez, Shelly C. Lu, José M. Mato, Maria L. MartíNez-Chantar

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

LKB1, originally considered a tumor suppressor, plays an important role in hepatocyte proliferation and liver regeneration. Mice lacking the methionine adenosyltransferase (MAT) gene MAT1A exhibit a chronic reduction in hepatic S-adenosylmethionine (SAMe) levels, basal activation of LKB1, and spontaneous development of nonalcoholic steatohepatitis (NASH) and hepatocellular carcinoma (HCC). These results are relevant for human health because patients with liver cirrhosis, who are at risk to develop HCC, have a marked reduction in hepatic MAT1A expression and SAMe synthesis. In this study, we isolated a cell line (SAMe-deficient [SAMe-D]) from MAT1A knockout (MAT1A-KO) mouse HCC to examine the role of LKB1 in the development of liver tumors derived from metabolic disorders. We found that LKB1 is required for cell survival in SAMe-D cells. LKB1 regulates Akt-mediated survival independent of phosphoinositide 3-kinase, adenosine monophosphate protein-activated kinase (AMPK), and mammalian target of rapamycin complex (mTORC2). In addition, LKB1 controls the apoptotic response through phosphorylation and retention of p53 in the cytoplasm and the regulation of herpesvirus-associated ubiquitin-specific protease (HAUSP) and Hu antigen R (HuR) nucleocytoplasmic shuttling. We identified HAUSP as a target of HuR. Finally, we observed cytoplasmic staining of p53 and p-LKB1(Ser428) in a NASH-HCC animal model (from MAT1A-KO mice) and in liver biopsies obtained from human HCC derived from both alcoholic steatohepatitis and NASH. Conclusion: The SAMe-D cell line is a relevant model of HCC derived from NASH disease in which LKB1 is the principal conductor of a new regulatory mechanism and could be a practical tool for uncovering new therapeutic strategies.

Original languageEnglish (US)
Pages (from-to)1621-1631
Number of pages11
JournalHepatology
Volume52
Issue number5
DOIs
StatePublished - Nov 2010
Externally publishedYes

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1-Phosphatidylinositol 4-Kinase
S-Adenosylmethionine
Hepatocellular Carcinoma
ELAV Proteins
Ubiquitin-Specific Proteases
Somatostatin-Secreting Cells
Herpesviridae
Liver
Knockout Mice
Methionine Adenosyltransferase
Alcoholic Fatty Liver
Cell Line
Liver Regeneration
Sirolimus
Adenosine Monophosphate
Liver Cirrhosis
Protein Kinases
Non-alcoholic Fatty Liver Disease
Hepatocytes
Neoplasms

ASJC Scopus subject areas

  • Hepatology

Cite this

MartíNez-LóPez, N., Varela-Rey, M., FernáNdez-Ramos, D., Woodhoo, A., VáZquez-Chantada, M., Embade, N., ... MartíNez-Chantar, M. L. (2010). Activation of LKB1-Akt pathway independent of phosphoinositide 3-kinase plays a critical role in the proliferation of hepatocellular carcinoma from nonalcoholic steatohepatitis. Hepatology, 52(5), 1621-1631. https://doi.org/10.1002/hep.23860

Activation of LKB1-Akt pathway independent of phosphoinositide 3-kinase plays a critical role in the proliferation of hepatocellular carcinoma from nonalcoholic steatohepatitis. / MartíNez-LóPez, Nuria; Varela-Rey, Marta; FernáNdez-Ramos, David; Woodhoo, Ashwin; VáZquez-Chantada, Mercedes; Embade, Nieves; Espinosa-Hevia, Luis; Bustamante, Francisco Javier; Parada, Luis A.; Rodriguez, Manuel S.; Lu, Shelly C.; Mato, José M.; MartíNez-Chantar, Maria L.

In: Hepatology, Vol. 52, No. 5, 11.2010, p. 1621-1631.

Research output: Contribution to journalArticle

MartíNez-LóPez, N, Varela-Rey, M, FernáNdez-Ramos, D, Woodhoo, A, VáZquez-Chantada, M, Embade, N, Espinosa-Hevia, L, Bustamante, FJ, Parada, LA, Rodriguez, MS, Lu, SC, Mato, JM & MartíNez-Chantar, ML 2010, 'Activation of LKB1-Akt pathway independent of phosphoinositide 3-kinase plays a critical role in the proliferation of hepatocellular carcinoma from nonalcoholic steatohepatitis', Hepatology, vol. 52, no. 5, pp. 1621-1631. https://doi.org/10.1002/hep.23860
MartíNez-LóPez, Nuria ; Varela-Rey, Marta ; FernáNdez-Ramos, David ; Woodhoo, Ashwin ; VáZquez-Chantada, Mercedes ; Embade, Nieves ; Espinosa-Hevia, Luis ; Bustamante, Francisco Javier ; Parada, Luis A. ; Rodriguez, Manuel S. ; Lu, Shelly C. ; Mato, José M. ; MartíNez-Chantar, Maria L. / Activation of LKB1-Akt pathway independent of phosphoinositide 3-kinase plays a critical role in the proliferation of hepatocellular carcinoma from nonalcoholic steatohepatitis. In: Hepatology. 2010 ; Vol. 52, No. 5. pp. 1621-1631.
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abstract = "LKB1, originally considered a tumor suppressor, plays an important role in hepatocyte proliferation and liver regeneration. Mice lacking the methionine adenosyltransferase (MAT) gene MAT1A exhibit a chronic reduction in hepatic S-adenosylmethionine (SAMe) levels, basal activation of LKB1, and spontaneous development of nonalcoholic steatohepatitis (NASH) and hepatocellular carcinoma (HCC). These results are relevant for human health because patients with liver cirrhosis, who are at risk to develop HCC, have a marked reduction in hepatic MAT1A expression and SAMe synthesis. In this study, we isolated a cell line (SAMe-deficient [SAMe-D]) from MAT1A knockout (MAT1A-KO) mouse HCC to examine the role of LKB1 in the development of liver tumors derived from metabolic disorders. We found that LKB1 is required for cell survival in SAMe-D cells. LKB1 regulates Akt-mediated survival independent of phosphoinositide 3-kinase, adenosine monophosphate protein-activated kinase (AMPK), and mammalian target of rapamycin complex (mTORC2). In addition, LKB1 controls the apoptotic response through phosphorylation and retention of p53 in the cytoplasm and the regulation of herpesvirus-associated ubiquitin-specific protease (HAUSP) and Hu antigen R (HuR) nucleocytoplasmic shuttling. We identified HAUSP as a target of HuR. Finally, we observed cytoplasmic staining of p53 and p-LKB1(Ser428) in a NASH-HCC animal model (from MAT1A-KO mice) and in liver biopsies obtained from human HCC derived from both alcoholic steatohepatitis and NASH. Conclusion: The SAMe-D cell line is a relevant model of HCC derived from NASH disease in which LKB1 is the principal conductor of a new regulatory mechanism and could be a practical tool for uncovering new therapeutic strategies.",
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T1 - Activation of LKB1-Akt pathway independent of phosphoinositide 3-kinase plays a critical role in the proliferation of hepatocellular carcinoma from nonalcoholic steatohepatitis

AU - MartíNez-LóPez, Nuria

AU - Varela-Rey, Marta

AU - FernáNdez-Ramos, David

AU - Woodhoo, Ashwin

AU - VáZquez-Chantada, Mercedes

AU - Embade, Nieves

AU - Espinosa-Hevia, Luis

AU - Bustamante, Francisco Javier

AU - Parada, Luis A.

AU - Rodriguez, Manuel S.

AU - Lu, Shelly C.

AU - Mato, José M.

AU - MartíNez-Chantar, Maria L.

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