Activation of endogenous Cdc42 visualized in living cells

Perihan Nalbant, Louis Hodgson, Vadim Kraynov, Alexei Toutchkine, Klaus M. Hahn

Research output: Contribution to journalArticlepeer-review

297 Scopus citations

Abstract

Signaling proteins are tightly regulated spatially and temporally to perform multiple functions. For Cdc42 and other guanosine triphosphatases, the subcellular location of activation is a critical determinant of cell behavior. However, current approaches are limited in their ability to examine the dynamics of Cdc42 activity in living cells. We report the development of a biosensor capable of visualizing the changing activation of endogenous, unlabeled Cdc42 in living cells. With the use of a dye that reports protein interactions, the biosensor revealed localized activation in the trans-Golgi apparatus, microtubule-dependent Cdc42 activation at the cell periphery, and activation kinetics precisely coordinated with cell extension and retraction.

Original languageEnglish (US)
Pages (from-to)1615-1619
Number of pages5
JournalScience
Volume305
Issue number5690
DOIs
StatePublished - Sep 10 2004
Externally publishedYes

ASJC Scopus subject areas

  • General

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