Activating the phosphate nucleophile at the catalytic site of purine nucleoside phosphorylase: A vibrational spectroscopic study

Hua Deng, Andrzej Lewandowicz, Vern L. Schramm, Robert Callender

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Abstract

Difference Raman and FTIR studies complemented by vibrational analysis based on ab initio calculations show that the dianionic phosphate in the PNP·ImmH·PO4 complex is forced into a unique bonding arrangement in which one of the P-O bonds is greatly polarized by enzyme active site interactions, such that it resembles a PO bond that is about one-quarter of the way toward forming a bridging P-O-C single P-O bond.

Original languageEnglish (US)
Pages (from-to)9516-9517
Number of pages2
JournalJournal of the American Chemical Society
Volume126
Issue number31
DOIs
Publication statusPublished - Aug 11 2004

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ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry

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