Activating photoactivatable proteins with laser light to visualize membrane systems and membrane traffic in living cells

Erik Lee Snapp, Patrick Lajoie

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

Eukaryotic cells are composed of an intricate system of internal membranes that are organized into different compartments-including the endoplasmic reticulum (ER), the nuclear envelope, the Golgi complex (GC), lysosomes, endosomes, caveolae, mitochondria, and peroxisomes-that perform specialized tasks within the cell. The localization and dynamics of intracellular compartments are now being studied in living cells because of the availability of green fluorescent protein (GFP)- fusion proteins and recent advances in fluorescent microscope imaging systems, such as the confocal laser-scanning microscope (CLSM). This protocol describes the steps for activating one of the first photoactivatable proteins, PA-GFP.

Original languageEnglish (US)
Pages (from-to)1368-1369
Number of pages2
JournalCold Spring Harbor Protocols
Volume6
Issue number11
DOIs
StatePublished - Nov 1 2011

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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