TY - JOUR
T1 - Activating photoactivatable proteins with laser light to visualize membrane systems and membrane traffic in living cells
AU - Snapp, Erik Lee
AU - Lajoie, Patrick
PY - 2011/11
Y1 - 2011/11
N2 - Eukaryotic cells are composed of an intricate system of internal membranes that are organized into different compartments-including the endoplasmic reticulum (ER), the nuclear envelope, the Golgi complex (GC), lysosomes, endosomes, caveolae, mitochondria, and peroxisomes-that perform specialized tasks within the cell. The localization and dynamics of intracellular compartments are now being studied in living cells because of the availability of green fluorescent protein (GFP)- fusion proteins and recent advances in fluorescent microscope imaging systems, such as the confocal laser-scanning microscope (CLSM). This protocol describes the steps for activating one of the first photoactivatable proteins, PA-GFP.
AB - Eukaryotic cells are composed of an intricate system of internal membranes that are organized into different compartments-including the endoplasmic reticulum (ER), the nuclear envelope, the Golgi complex (GC), lysosomes, endosomes, caveolae, mitochondria, and peroxisomes-that perform specialized tasks within the cell. The localization and dynamics of intracellular compartments are now being studied in living cells because of the availability of green fluorescent protein (GFP)- fusion proteins and recent advances in fluorescent microscope imaging systems, such as the confocal laser-scanning microscope (CLSM). This protocol describes the steps for activating one of the first photoactivatable proteins, PA-GFP.
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U2 - 10.1101/pdb.prot066571
DO - 10.1101/pdb.prot066571
M3 - Article
C2 - 22046039
AN - SCOPUS:80455127022
SN - 1559-6095
VL - 6
SP - 1368
EP - 1369
JO - Cold Spring Harbor Protocols
JF - Cold Spring Harbor Protocols
IS - 11
ER -