Activating photoactivatable proteins with laser light to visualize membrane systems and membrane traffic in living cells

Erik Lee Snapp, Patrick Lajoie

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Eukaryotic cells are composed of an intricate system of internal membranes that are organized into different compartments-including the endoplasmic reticulum (ER), the nuclear envelope, the Golgi complex (GC), lysosomes, endosomes, caveolae, mitochondria, and peroxisomes-that perform specialized tasks within the cell. The localization and dynamics of intracellular compartments are now being studied in living cells because of the availability of green fluorescent protein (GFP)- fusion proteins and recent advances in fluorescent microscope imaging systems, such as the confocal laser-scanning microscope (CLSM). This protocol describes the steps for activating one of the first photoactivatable proteins, PA-GFP.

Original languageEnglish (US)
Pages (from-to)1368-1369
Number of pages2
JournalCold Spring Harbor Protocols
Volume6
Issue number11
DOIs
StatePublished - Nov 2011

Fingerprint

Green Fluorescent Proteins
Telecommunication traffic
Lasers
Microscopes
Cells
Membranes
Light
Caveolae
Mitochondria
Peroxisomes
Endosomes
Nuclear Envelope
Golgi Apparatus
Eukaryotic Cells
Lysosomes
Endoplasmic Reticulum
Imaging systems
Proteins
Fusion reactions
Availability

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Activating photoactivatable proteins with laser light to visualize membrane systems and membrane traffic in living cells. / Snapp, Erik Lee; Lajoie, Patrick.

In: Cold Spring Harbor Protocols, Vol. 6, No. 11, 11.2011, p. 1368-1369.

Research output: Contribution to journalArticle

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