Action of light and streptomycin on protein synthesis in cabbage seedlings

Research output: Contribution to journalArticle

Abstract

The action of light on protein synthesis was examined in the cabbage seedlings, a system extensively used in the studies of anthocyanin synthesis. Continuous red and far red light have no effect on total protein content while they cause a marked decrease in the level of free amino acids in cabbage seedlings. The rate of protein synthesis, measured as incorporation of radioaetively-labelled amino acids into proteins, is clearly stimulated by light. Phytochrome involvement in the light stimulation of the incorporation is also demonstrated by the red-far red reversibility of the response. The relative effectiveness of continuous red and far red light upon the incorporation of amino acids into proteins is affected by the nature of the system used to study the incorporation process. When excised cotyledons and short period of incorporation were used, continuous far red was more effective than red. However, when whole seedlings and long period of incorporation were used, red and far red were equally effective. Streptomycin causes a 10- 15% decrease in the rate of incorporation of amino acids into proteins of all cellular fractions, except the plastid fraction where a much higher inhibition (30%) was observed.

Original languageEnglish (US)
Pages (from-to)296-302
Number of pages7
JournalBiologia Plantarum
Volume24
Issue number4
DOIs
StatePublished - Jul 1982
Externally publishedYes

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streptomycin
cabbage
protein synthesis
far-red light
amino acids
seedlings
proteins
phytochrome
free amino acids
plastids
cotyledons
anthocyanins
protein content
synthesis

ASJC Scopus subject areas

  • Plant Science
  • Horticulture

Cite this

Action of light and streptomycin on protein synthesis in cabbage seedlings. / Yang, Chia-Ping H.

In: Biologia Plantarum, Vol. 24, No. 4, 07.1982, p. 296-302.

Research output: Contribution to journalArticle

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