Actin filaments are involved in the maintenance of Golgi cisternae morphology and intra-Golgi pH

Francisco Lázaro-Diéguez, Nuria Jiménez, Holger Barth, Abraham J. Koster, Jaime Renau-Piqueras, Juan L. Llopis, Koert N.J. Burger, Gustavo Egea

Research output: Contribution to journalArticle

41 Scopus citations

Abstract

Here we examine the contribution of actin dynamics to the architecture and pH of the Golgi complex. To this end, we have used toxins that depolymerize (cytochalasin D, latrunculin B, mycalolide B, and Clostridium botulinum C2 toxin) or stabilize (jasplakinolide) filamentous actin. When various clonal cell lines were examined by epifluorescence microscopy, all of these actin toxins induced compaction of the Golgi complex. However, ultrastructural analysis by transmission electron microscopy and electron tomography/three-dimensional modelling of the Golgi complex showed that F-actin depolymerization first induces perforation/fragmentation and severe swelling of Golgi cisternae, which leads to a completely disorganized structure. In contrast, F-actin stabilization results only in cisternae perforation/fragmentation. Concomitantly to actin depolymerization-induced cisternae swelling and disorganization, the intra-Golgi pH significantly increased. Similar ultrastructural and Golgi pH alkalinization were observed in cells treated with the vacuolar H+-ATPases inhibitors bafilomycin A1 and concanamycin A. Overall, these results suggest that actin filaments are implicated in the preservation of the flattened shape of Golgi cisternae. This maintenance seems to be mediated by the regulation of the state of F-actin assembly on the Golgi pH homeostasis.

Original languageEnglish (US)
Pages (from-to)778-791
Number of pages14
JournalCell motility and the cytoskeleton
Volume63
Issue number12
DOIs
StatePublished - Dec 1 2006
Externally publishedYes

Keywords

  • Actin
  • Cytoskeleton
  • Electron tomography
  • Golgi apparatus
  • pH

ASJC Scopus subject areas

  • Structural Biology
  • Cell Biology

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    Lázaro-Diéguez, F., Jiménez, N., Barth, H., Koster, A. J., Renau-Piqueras, J., Llopis, J. L., Burger, K. N. J., & Egea, G. (2006). Actin filaments are involved in the maintenance of Golgi cisternae morphology and intra-Golgi pH. Cell motility and the cytoskeleton, 63(12), 778-791. https://doi.org/10.1002/cm.20161