Actin dynamics in lamellipodia of migrating border cells in the Drosophila ovary revealed by a GFP-actin fusion protein

Vladislav Verkhusha, Shoichiro Tsukita, Hiroki Oda

Research output: Contribution to journalArticle

94 Citations (Scopus)

Abstract

Directional migration of border cells in the Drosophila egg chambers is a developmentally regulated event that requires dynamic cellular functions. In this study, the electron microscopic observation of migrating border cells revealed loose actin bundles in forepart lamellipodia and numerous microvilli extending from nurse cells and providing multiple adhesive contacts with border cells. To analyze the dynamics of actin in migrating border cells in vivo, we constructed a green fluorescent protein-actin fusion protein and induced its expression in Drosophila using the GAL4/UAS system. The green fluorescent protein-actin was incorporated into the actin bundles and it enabled visualization of the rapid cytoskeletal changes in border cell lamellipodia. During the growth of the lamellipodia, the actin bundles that increased in number and size radiated from the bundle-organizing center. Quantification of the fluorescence intensity showed that an accumulation of bundle-associated and spotted green fluorescent protein-actin signals took place during their centripetal movement. Our results favored a treadmilling model for actin behavior in border cell lamellipodia. Copyright (C) 1999 Federation of European Biochemical Societies.

Original languageEnglish (US)
Pages (from-to)395-401
Number of pages7
JournalFEBS Letters
Volume445
Issue number2-3
DOIs
StatePublished - Feb 26 1999
Externally publishedYes

Fingerprint

Pseudopodia
Drosophila
Actins
Ovary
Fusion reactions
Proteins
Green Fluorescent Proteins
Microvilli
Adhesives
Cell Movement
Ovum
Visualization
Fluorescence
Nurses
Electrons
Growth

Keywords

  • Actin dynamics
  • BC, border cell
  • BDM, 2,3-butanedione monoxime
  • BOC, bundle-organizing center
  • Border cell migration
  • Forepart lamellipodium
  • GFP, green fluorescent protein
  • Green fluorescent protein-actin fusion
  • NC, nurse cell

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Actin dynamics in lamellipodia of migrating border cells in the Drosophila ovary revealed by a GFP-actin fusion protein. / Verkhusha, Vladislav; Tsukita, Shoichiro; Oda, Hiroki.

In: FEBS Letters, Vol. 445, No. 2-3, 26.02.1999, p. 395-401.

Research output: Contribution to journalArticle

@article{6c3aa1e75c674d3d9f4bb81d468e41fe,
title = "Actin dynamics in lamellipodia of migrating border cells in the Drosophila ovary revealed by a GFP-actin fusion protein",
abstract = "Directional migration of border cells in the Drosophila egg chambers is a developmentally regulated event that requires dynamic cellular functions. In this study, the electron microscopic observation of migrating border cells revealed loose actin bundles in forepart lamellipodia and numerous microvilli extending from nurse cells and providing multiple adhesive contacts with border cells. To analyze the dynamics of actin in migrating border cells in vivo, we constructed a green fluorescent protein-actin fusion protein and induced its expression in Drosophila using the GAL4/UAS system. The green fluorescent protein-actin was incorporated into the actin bundles and it enabled visualization of the rapid cytoskeletal changes in border cell lamellipodia. During the growth of the lamellipodia, the actin bundles that increased in number and size radiated from the bundle-organizing center. Quantification of the fluorescence intensity showed that an accumulation of bundle-associated and spotted green fluorescent protein-actin signals took place during their centripetal movement. Our results favored a treadmilling model for actin behavior in border cell lamellipodia. Copyright (C) 1999 Federation of European Biochemical Societies.",
keywords = "Actin dynamics, BC, border cell, BDM, 2,3-butanedione monoxime, BOC, bundle-organizing center, Border cell migration, Forepart lamellipodium, GFP, green fluorescent protein, Green fluorescent protein-actin fusion, NC, nurse cell",
author = "Vladislav Verkhusha and Shoichiro Tsukita and Hiroki Oda",
year = "1999",
month = "2",
day = "26",
doi = "10.1016/S0014-5793(99)00124-6",
language = "English (US)",
volume = "445",
pages = "395--401",
journal = "FEBS Letters",
issn = "0014-5793",
publisher = "Elsevier",
number = "2-3",

}

TY - JOUR

T1 - Actin dynamics in lamellipodia of migrating border cells in the Drosophila ovary revealed by a GFP-actin fusion protein

AU - Verkhusha, Vladislav

AU - Tsukita, Shoichiro

AU - Oda, Hiroki

PY - 1999/2/26

Y1 - 1999/2/26

N2 - Directional migration of border cells in the Drosophila egg chambers is a developmentally regulated event that requires dynamic cellular functions. In this study, the electron microscopic observation of migrating border cells revealed loose actin bundles in forepart lamellipodia and numerous microvilli extending from nurse cells and providing multiple adhesive contacts with border cells. To analyze the dynamics of actin in migrating border cells in vivo, we constructed a green fluorescent protein-actin fusion protein and induced its expression in Drosophila using the GAL4/UAS system. The green fluorescent protein-actin was incorporated into the actin bundles and it enabled visualization of the rapid cytoskeletal changes in border cell lamellipodia. During the growth of the lamellipodia, the actin bundles that increased in number and size radiated from the bundle-organizing center. Quantification of the fluorescence intensity showed that an accumulation of bundle-associated and spotted green fluorescent protein-actin signals took place during their centripetal movement. Our results favored a treadmilling model for actin behavior in border cell lamellipodia. Copyright (C) 1999 Federation of European Biochemical Societies.

AB - Directional migration of border cells in the Drosophila egg chambers is a developmentally regulated event that requires dynamic cellular functions. In this study, the electron microscopic observation of migrating border cells revealed loose actin bundles in forepart lamellipodia and numerous microvilli extending from nurse cells and providing multiple adhesive contacts with border cells. To analyze the dynamics of actin in migrating border cells in vivo, we constructed a green fluorescent protein-actin fusion protein and induced its expression in Drosophila using the GAL4/UAS system. The green fluorescent protein-actin was incorporated into the actin bundles and it enabled visualization of the rapid cytoskeletal changes in border cell lamellipodia. During the growth of the lamellipodia, the actin bundles that increased in number and size radiated from the bundle-organizing center. Quantification of the fluorescence intensity showed that an accumulation of bundle-associated and spotted green fluorescent protein-actin signals took place during their centripetal movement. Our results favored a treadmilling model for actin behavior in border cell lamellipodia. Copyright (C) 1999 Federation of European Biochemical Societies.

KW - Actin dynamics

KW - BC, border cell

KW - BDM, 2,3-butanedione monoxime

KW - BOC, bundle-organizing center

KW - Border cell migration

KW - Forepart lamellipodium

KW - GFP, green fluorescent protein

KW - Green fluorescent protein-actin fusion

KW - NC, nurse cell

UR - http://www.scopus.com/inward/record.url?scp=0033052254&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033052254&partnerID=8YFLogxK

U2 - 10.1016/S0014-5793(99)00124-6

DO - 10.1016/S0014-5793(99)00124-6

M3 - Article

VL - 445

SP - 395

EP - 401

JO - FEBS Letters

JF - FEBS Letters

SN - 0014-5793

IS - 2-3

ER -