Actin crosslinking protein EF-1 a of Dictyostelium discoideum has a unique bonding rule that allows square-packed bundles

Cameron H. Owen, David J. Derosier, John Condeelis

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Abstract

The elongation factor 1a (EF-1a) of Dictyostelium discoideum is an actin crosslinking protein that gives rise to a unique kind of actin bundle. Purified actin and EF-1a were allowed to form bundles and then were characterized by electron microscopy, computed diffraction analysis, and modeling. In these bundles crosslinked actin filaments are rotated by 90° relative to each other, whereas other known crosslinking proteins require filaments to be unrotated. Bundles of actin EF-1a would tend to exclude other actin bundling proteins. EF-1a can thus regulate the state of the actin cytoskeleton as well as regulate protein synthesis.

Original languageEnglish (US)
Pages (from-to)248-254
Number of pages7
JournalJournal of Structural Biology
Volume109
Issue number3
DOIs
Publication statusPublished - Jan 1 1992

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ASJC Scopus subject areas

  • Structural Biology

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