Actin crosslinking protein EF-1 a of Dictyostelium discoideum has a unique bonding rule that allows square-packed bundles

Cameron H. Owen, David J. Derosier, John S. Condeelis

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

The elongation factor 1a (EF-1a) of Dictyostelium discoideum is an actin crosslinking protein that gives rise to a unique kind of actin bundle. Purified actin and EF-1a were allowed to form bundles and then were characterized by electron microscopy, computed diffraction analysis, and modeling. In these bundles crosslinked actin filaments are rotated by 90° relative to each other, whereas other known crosslinking proteins require filaments to be unrotated. Bundles of actin EF-1a would tend to exclude other actin bundling proteins. EF-1a can thus regulate the state of the actin cytoskeleton as well as regulate protein synthesis.

Original languageEnglish (US)
Pages (from-to)248-254
Number of pages7
JournalJournal of Structural Biology
Volume109
Issue number3
DOIs
StatePublished - 1992

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Peptide Elongation Factor 1
Dictyostelium
Peptide Elongation Factors
Actins
Proteins
Actin Cytoskeleton
Transmission Electron Microscopy

ASJC Scopus subject areas

  • Structural Biology

Cite this

Actin crosslinking protein EF-1 a of Dictyostelium discoideum has a unique bonding rule that allows square-packed bundles. / Owen, Cameron H.; Derosier, David J.; Condeelis, John S.

In: Journal of Structural Biology, Vol. 109, No. 3, 1992, p. 248-254.

Research output: Contribution to journalArticle

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