Actin-containing microprocesses in the fusion of cultured chick myoblasts.

H. L. Huang, Robert H. Singer, E. Lazarides

Research output: Contribution to journalArticle

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Abstract

Scanning electron microscopic studies of myoblasts from 11- to 13-day-old chick embroyonic breast muscle cultured on collagen-coated glass coverslips showed six stages of development into multinucleated myotubes: (1) growth of flattened, spread-out cells for 20-30 hr following initiation of monolayer cultures; (2) extension of microprocesses (1-150 microM) from cells that have become spindle shaped; (3) contact and adherence of microprocesses from adjacent cells; (4) thickening of fused processes; (5) approximation of the cells; and (6) coalescence of the cells to form a spindle-shaped myotube. When the calcium-ion concentration in the growth medium was lowered--either by increasing the concentration of ethylene-glycol-bis(aminoethyl ether)N,N'-tetraacetate (EGTA)or by decreasing the cconcentration of free calciumion used--the number of microprocesses present on the cells was reduced. Presumably, however, these microprocesses could still fuse together, provided that the calciumion concentration was greater than 160 microM. Indirect immunofluorescence assay with actin-specific antibody indicated that actin is a major component of the myoblasts' microprocesses. Cytochalasin B (5 microgram/ml) caused the microprocesses to retract within 15 min and the myoblasts to round up and detach from the glass substrate. This was presumably caused by the action of the drug on actin filaments.

Original languageEnglish (US)
Pages (from-to)219-229
Number of pages11
JournalMuscle and Nerve
Volume1
Issue number3
StatePublished - May 1978
Externally publishedYes

Fingerprint

Myoblasts
Actins
Skeletal Muscle Fibers
Glass
Cytochalasin B
Ethylene Glycol
Egtazic Acid
Indirect Fluorescent Antibody Technique
Growth
Actin Cytoskeleton
Ether
Breast
Collagen
Electrons
Ions
Calcium
Muscles
Antibodies
Pharmaceutical Preparations

ASJC Scopus subject areas

  • Clinical Neurology
  • Neuroscience(all)

Cite this

Actin-containing microprocesses in the fusion of cultured chick myoblasts. / Huang, H. L.; Singer, Robert H.; Lazarides, E.

In: Muscle and Nerve, Vol. 1, No. 3, 05.1978, p. 219-229.

Research output: Contribution to journalArticle

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