Acquisition of increased hormone sensitivity during in vitro adipocyte development

C. S. Rubin, E. Lai, O. M. Rosen

Research output: Contribution to journalArticle

136 Citations (Scopus)

Abstract

Murine 3T3 L1 fibroblasts enter a differentiation program subsequent to prolonged maintenance in the confluent state and develop into adipocytes. The hormone sensitivity of adenylate cyclase and the physiological responsiveness to insulin were compared in 3T3 L1 preadipocytes and adipocytes. The following observations, comprising several distinct categories of hormone responsiveness, were made. (2.5 μM) isoproterenol stimulated adenylate cyclase 15 fold in adipocyte homogenates, but only 2.5 fold in preadipocyte preparations, suggesting a considerable magnification in β adrenergic responsiveness during development. A totally new control element, adrenocorticotropic hormone responsiveness, was incorporated into the adenylate cyclase system of the adipocytes. Sensitivity to prostaglandin E1 was observed in both preadipocytes and adipocytes, but no change in responsiveness could be detected in the differentiated cells. Glucagon sensitive adenylate cyclase could not be detected in either preadipocytes or adipocytes. Both preadipocytes and adipocytes possess considerable insulin binding activity, but near physiological levels of insulin stimulate the conversion of glucose to CO2 and lipid only in the differentiated cells.

Original languageEnglish (US)
Pages (from-to)3554-3557
Number of pages4
JournalJournal of Biological Chemistry
Volume252
Issue number10
StatePublished - 1977

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Adenylyl Cyclases
Adipocytes
Hormones
Insulin
Alprostadil
Fibroblasts
Glucagon
Isoproterenol
Adrenergic Agents
Adrenocorticotropic Hormone
Lipids
Glucose
In Vitro Techniques
Maintenance

ASJC Scopus subject areas

  • Biochemistry

Cite this

Acquisition of increased hormone sensitivity during in vitro adipocyte development. / Rubin, C. S.; Lai, E.; Rosen, O. M.

In: Journal of Biological Chemistry, Vol. 252, No. 10, 1977, p. 3554-3557.

Research output: Contribution to journalArticle

Rubin, C. S. ; Lai, E. ; Rosen, O. M. / Acquisition of increased hormone sensitivity during in vitro adipocyte development. In: Journal of Biological Chemistry. 1977 ; Vol. 252, No. 10. pp. 3554-3557.
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