Acidosis-induced metallothionein (MT) mRNA expression in neonatal rat primary astrocyte cultures

Michael Aschner, S. Stark, K. E. Vrana, D. R. Conklin

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Metallothionein (MT) mRNA levels were determined following exposure of neonatal rat primary astrocyte cultures to acidosis. Astrocyte total RNA was probed on northern blots with [α32P]dCTP-labeled synthetic cDNA probes specific for rat MT isoform mRNAs. The probe for MT-I mRNA hybridized to a single mRNA with a size appropriate for MT, approximately 550 nucleotides. MT-I mRNA levels in astrocyte monolayers exposed to pH 6.5 and 6.0 for 3 or 6 hours were unchanged compared with MT-I mRNA levels in control cultures exposed to pH 7.4. In contrast, 9 hour exposure of astrocytes to pH 6.5 and 6.0 led to a significant increase in MT-I mRNA transcripts compared with controls maintained at pH 7.4 (p<0.001 and p<0.02, respectively). A probe for MT-II mRNA that hybridizes to a single mRNA (450 nucleotides) was also used to determine the effect of acidosis on astrocyte MT-II mRNA transcripts. Although statistical significance was not attained, a similar trend was noted, with a 9 hour exposure to pH of 6.5 and 6.0 resulting in increased astrocytic expression MT-II mRNA compared with control cells maintained at pH 7.4. Acidosis was also associated with a pH-dependent increase in astrocytic volume. Accordingly, acidosis is invoked as an added stimulus to stress factors associated with the induction of astrocytic MT mRNA transcripts.

Original languageEnglish (US)
Pages (from-to)227-236
Number of pages10
JournalNeuroToxicology
Volume19
Issue number2
StatePublished - 1998
Externally publishedYes

Fingerprint

Metallothionein
Acidosis
Astrocytes
Rats
Messenger RNA
Nucleotides
RNA Isoforms
Northern Blotting
Monolayers
Complementary DNA
RNA

Keywords

  • Acidosis
  • Astrocytes
  • Cell Volume
  • In vitro
  • Metallothionein
  • mRNA

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Neuroscience(all)
  • Toxicology

Cite this

Acidosis-induced metallothionein (MT) mRNA expression in neonatal rat primary astrocyte cultures. / Aschner, Michael; Stark, S.; Vrana, K. E.; Conklin, D. R.

In: NeuroToxicology, Vol. 19, No. 2, 1998, p. 227-236.

Research output: Contribution to journalArticle

Aschner, Michael ; Stark, S. ; Vrana, K. E. ; Conklin, D. R. / Acidosis-induced metallothionein (MT) mRNA expression in neonatal rat primary astrocyte cultures. In: NeuroToxicology. 1998 ; Vol. 19, No. 2. pp. 227-236.
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