Acidic stress promotes a glioma stem cell phenotype

A. B. Hjelmeland, Q. Wu, J. M. Heddleston, G. S. Choudhary, J. MacSwords, J. D. Lathia, R. McLendon, D. Lindner, A. Sloan, J. N. Rich

Research output: Contribution to journalArticlepeer-review

242 Scopus citations

Abstract

Malignant gliomas are lethal cancers that display cellular hierarchies with cancer stem cells at the apex. Glioma stem cells (GSCs) are not uniformly distributed, but rather located in specialized niches, suggesting that the cancer stem cell phenotype is regulated by the tumor microenvironment. Indeed, recent studies show that hypoxia and its molecular responses regulate cancer stem cell maintenance. We now demonstrate that acidic conditions, independent of restricted oxygen, promote the expression of GSC markers, self-renewal and tumor growth. GSCs exert paracrine effects on tumor growth through elaboration of angiogenic factors, and low pH conditions augment this expression associated with induction of hypoxia inducible factor 2α (HIF2α), a GSC-specific regulator. Induction of HIF2α and other GSC markers by acidic stress can be reverted by elevating pH in vitro, suggesting that raising intratumoral pH may be beneficial for targeting the GSC phenotype. Together, our results suggest that exposure to low pH promotes malignancy through the induction of a cancer stem cell phenotype, and that culturing cancer cells at lower pH reflective of endogenous tumor conditions may better retain the cellular heterogeneity found in tumors.

Original languageEnglish (US)
Pages (from-to)829-840
Number of pages12
JournalCell Death and Differentiation
Volume18
Issue number5
DOIs
StatePublished - May 2011
Externally publishedYes

Keywords

  • acidic microenvironment
  • cancer stem cell
  • glioma
  • pH

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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