Accurate identification of single-nucleotide variants in whole-genome-amplified single cells

Xiao Dong, Lei Zhang, Brandon Milholland, Moonsook Lee, Alexander Y. Maslov, Tao Wang, Jan Vijg

Research output: Research - peer-reviewArticle

  • 7 Citations

Abstract

Mutation analysis in single-cell genomes is prone to artifacts associated with cell lysis and whole-genome amplification. Here we addressed these issues by developing single-cell multiple displacement amplification (SCMDA) and a general-purpose single-cell-variant caller, SCcaller (https://github.com/biosinodx/SCcaller/). By comparing SCMDA-amplified single cells with unamplified clones from the same population, we validated the procedure as a firm foundation for standardized somatic-mutation analysis in single-cell genomics.

LanguageEnglish (US)
JournalNature Methods
DOIs
StateAccepted/In press - Mar 20 2017

Fingerprint

Nucleotides
Genome
Amplification
Genes
Single-Cell Analysis
Mutation
Genomics
Artifacts
Clone Cells
Population

ASJC Scopus subject areas

  • Biotechnology
  • Molecular Biology
  • Biochemistry
  • Cell Biology

Cite this

Accurate identification of single-nucleotide variants in whole-genome-amplified single cells. / Dong, Xiao; Zhang, Lei; Milholland, Brandon; Lee, Moonsook; Maslov, Alexander Y.; Wang, Tao; Vijg, Jan.

In: Nature Methods, 20.03.2017.

Research output: Research - peer-reviewArticle

Dong X, Zhang L, Milholland B, Lee M, Maslov AY, Wang T et al. Accurate identification of single-nucleotide variants in whole-genome-amplified single cells. Nature Methods. 2017 Mar 20. Available from, DOI: 10.1038/nmeth.4227
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