Absence of transepithelial anion exchange by rabbit OMCD

Evidence against reversal of cell polarity

M. Hayashi, Victor L. Schuster, J. B. Stokes

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

In the rabbit cortical collecting duct (CCD), Cl tracer crosses the epithelium predominantly via an anion exchange system that operates in either a Cl-Cl or Cl-HCO3 exchange mode. In the present study, we used the 36Cl lumen-to-bath rate coefficient (K(Cl), nm/s), a sensitive measurement of CCD transepithelial anion transport, to investigate the nature of Cl transport in the medullary collecting duct dissected from inner stripe, outer medulla (OMCD). The K(Cl) in OMCD perfused and bathed in HCO3-Ringer solution was low (46.2 ± 8.5 nm/s) and similar to that value observed in the CCD when anion exchange is inhibited and Cl permeates the epithelium by diffusion. Unlike K(Cl) in CCD, K(Cl) in OMCD was not stimulated by adenosine 3',5'-cyclic monophosphate (cAMP). OMCD K(Cl) was not altered by bath Cl and/or HCO3 removal, demonstrating the absence of transepithelial Cl-Cl and Cl-HCO3 exchange. To test the hypothesis that metabolic alkalosis could reverse the polarity of intercalated cells and thus induce an apical Cl-HCO3 exchanger in H+-secreting OMCD cells, we measured K(Cl) in OMCD from rabbits made alkalotic by deoxycorticosterone and furosemide. Although the base-line K(Cl) was slightly higher than in OMCD from control rabbits, the value was still far lower than the K(Cl) under comparable conditions in CCD. Moreover, K(Cl) in OMCD from alkalotic rabbits was unchanged by cAMP, or by sequential removal of bath HCO3 and Cl. Immunocytochemistry using peanut lectin and a monoclonal antibody to erythrocyte band 3 failed to reveal any evidence for alkalosis-induced reversal of either CCD or OMCD intercalated cell polarity. We conclude 1) Cl transport across the MCD by anion exchange is immeasurably low or nonexistent; 2) unlike the CCD, Cl transport in OMCD is not responsive to cAMP; and 3) metabolic alkalosis does not induce an apical anion exchanger in OMCD, i.e., does not cause epithelial polarity reversal.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Renal Fluid and Electrolyte Physiology
Volume255
Issue number2
StatePublished - 1988
Externally publishedYes

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Cell Polarity
Anions
Alkalosis
Rabbits
Baths
Epithelium
Chloride-Bicarbonate Antiporters
Peanut Agglutinin
Desoxycorticosterone
Furosemide
Cyclic AMP
Erythrocytes
Immunohistochemistry
Monoclonal Antibodies

ASJC Scopus subject areas

  • Physiology

Cite this

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title = "Absence of transepithelial anion exchange by rabbit OMCD: Evidence against reversal of cell polarity",
abstract = "In the rabbit cortical collecting duct (CCD), Cl tracer crosses the epithelium predominantly via an anion exchange system that operates in either a Cl-Cl or Cl-HCO3 exchange mode. In the present study, we used the 36Cl lumen-to-bath rate coefficient (K(Cl), nm/s), a sensitive measurement of CCD transepithelial anion transport, to investigate the nature of Cl transport in the medullary collecting duct dissected from inner stripe, outer medulla (OMCD). The K(Cl) in OMCD perfused and bathed in HCO3-Ringer solution was low (46.2 ± 8.5 nm/s) and similar to that value observed in the CCD when anion exchange is inhibited and Cl permeates the epithelium by diffusion. Unlike K(Cl) in CCD, K(Cl) in OMCD was not stimulated by adenosine 3',5'-cyclic monophosphate (cAMP). OMCD K(Cl) was not altered by bath Cl and/or HCO3 removal, demonstrating the absence of transepithelial Cl-Cl and Cl-HCO3 exchange. To test the hypothesis that metabolic alkalosis could reverse the polarity of intercalated cells and thus induce an apical Cl-HCO3 exchanger in H+-secreting OMCD cells, we measured K(Cl) in OMCD from rabbits made alkalotic by deoxycorticosterone and furosemide. Although the base-line K(Cl) was slightly higher than in OMCD from control rabbits, the value was still far lower than the K(Cl) under comparable conditions in CCD. Moreover, K(Cl) in OMCD from alkalotic rabbits was unchanged by cAMP, or by sequential removal of bath HCO3 and Cl. Immunocytochemistry using peanut lectin and a monoclonal antibody to erythrocyte band 3 failed to reveal any evidence for alkalosis-induced reversal of either CCD or OMCD intercalated cell polarity. We conclude 1) Cl transport across the MCD by anion exchange is immeasurably low or nonexistent; 2) unlike the CCD, Cl transport in OMCD is not responsive to cAMP; and 3) metabolic alkalosis does not induce an apical anion exchanger in OMCD, i.e., does not cause epithelial polarity reversal.",
author = "M. Hayashi and Schuster, {Victor L.} and Stokes, {J. B.}",
year = "1988",
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T1 - Absence of transepithelial anion exchange by rabbit OMCD

T2 - Evidence against reversal of cell polarity

AU - Hayashi, M.

AU - Schuster, Victor L.

AU - Stokes, J. B.

PY - 1988

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N2 - In the rabbit cortical collecting duct (CCD), Cl tracer crosses the epithelium predominantly via an anion exchange system that operates in either a Cl-Cl or Cl-HCO3 exchange mode. In the present study, we used the 36Cl lumen-to-bath rate coefficient (K(Cl), nm/s), a sensitive measurement of CCD transepithelial anion transport, to investigate the nature of Cl transport in the medullary collecting duct dissected from inner stripe, outer medulla (OMCD). The K(Cl) in OMCD perfused and bathed in HCO3-Ringer solution was low (46.2 ± 8.5 nm/s) and similar to that value observed in the CCD when anion exchange is inhibited and Cl permeates the epithelium by diffusion. Unlike K(Cl) in CCD, K(Cl) in OMCD was not stimulated by adenosine 3',5'-cyclic monophosphate (cAMP). OMCD K(Cl) was not altered by bath Cl and/or HCO3 removal, demonstrating the absence of transepithelial Cl-Cl and Cl-HCO3 exchange. To test the hypothesis that metabolic alkalosis could reverse the polarity of intercalated cells and thus induce an apical Cl-HCO3 exchanger in H+-secreting OMCD cells, we measured K(Cl) in OMCD from rabbits made alkalotic by deoxycorticosterone and furosemide. Although the base-line K(Cl) was slightly higher than in OMCD from control rabbits, the value was still far lower than the K(Cl) under comparable conditions in CCD. Moreover, K(Cl) in OMCD from alkalotic rabbits was unchanged by cAMP, or by sequential removal of bath HCO3 and Cl. Immunocytochemistry using peanut lectin and a monoclonal antibody to erythrocyte band 3 failed to reveal any evidence for alkalosis-induced reversal of either CCD or OMCD intercalated cell polarity. We conclude 1) Cl transport across the MCD by anion exchange is immeasurably low or nonexistent; 2) unlike the CCD, Cl transport in OMCD is not responsive to cAMP; and 3) metabolic alkalosis does not induce an apical anion exchanger in OMCD, i.e., does not cause epithelial polarity reversal.

AB - In the rabbit cortical collecting duct (CCD), Cl tracer crosses the epithelium predominantly via an anion exchange system that operates in either a Cl-Cl or Cl-HCO3 exchange mode. In the present study, we used the 36Cl lumen-to-bath rate coefficient (K(Cl), nm/s), a sensitive measurement of CCD transepithelial anion transport, to investigate the nature of Cl transport in the medullary collecting duct dissected from inner stripe, outer medulla (OMCD). The K(Cl) in OMCD perfused and bathed in HCO3-Ringer solution was low (46.2 ± 8.5 nm/s) and similar to that value observed in the CCD when anion exchange is inhibited and Cl permeates the epithelium by diffusion. Unlike K(Cl) in CCD, K(Cl) in OMCD was not stimulated by adenosine 3',5'-cyclic monophosphate (cAMP). OMCD K(Cl) was not altered by bath Cl and/or HCO3 removal, demonstrating the absence of transepithelial Cl-Cl and Cl-HCO3 exchange. To test the hypothesis that metabolic alkalosis could reverse the polarity of intercalated cells and thus induce an apical Cl-HCO3 exchanger in H+-secreting OMCD cells, we measured K(Cl) in OMCD from rabbits made alkalotic by deoxycorticosterone and furosemide. Although the base-line K(Cl) was slightly higher than in OMCD from control rabbits, the value was still far lower than the K(Cl) under comparable conditions in CCD. Moreover, K(Cl) in OMCD from alkalotic rabbits was unchanged by cAMP, or by sequential removal of bath HCO3 and Cl. Immunocytochemistry using peanut lectin and a monoclonal antibody to erythrocyte band 3 failed to reveal any evidence for alkalosis-induced reversal of either CCD or OMCD intercalated cell polarity. We conclude 1) Cl transport across the MCD by anion exchange is immeasurably low or nonexistent; 2) unlike the CCD, Cl transport in OMCD is not responsive to cAMP; and 3) metabolic alkalosis does not induce an apical anion exchanger in OMCD, i.e., does not cause epithelial polarity reversal.

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