Absence of mouse mammary tumor virus proviral amplification in chemically induced lymphomas of RF/J mice

R. Kraft, S. T. Ishizaka, S. A. Okenquist, Geoffrey J. Childs, F. Lilly, Jack Lenz

Research output: Contribution to journalArticle

Abstract

RF/J mice are susceptible to the induction of thymic lymphomas by the carcinogens 3-methylcholanthrene and N-methyl-N-nitrosourea. Given the association of mouse mammary tumor virus (MMTV) with certain thymomas, we examined genomic DNA from chemically induced lymphomas of RF/J mice for new MMTV proviruses. Of 13 tissue culture lines derived from 3-methylcholanthrene-induced tumors, 5 had acquired new proviruses. MMTV amplification coincided with the appearance of viral mRNAs and proteins. However, no primary tumors or animal-passaged tumors contained new proviruses. These observations indicate that MMTV does not have a role in the tumor induction process, although it may become activated and amplified in tissue culture lines derived from tumors.

Original languageEnglish (US)
Pages (from-to)3200-3204
Number of pages5
JournalJournal of Virology
Volume63
Issue number7
StatePublished - 1989

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Mouse mammary tumor virus
lymphoma
Lymphoma
proviruses
Proviruses
neoplasms
mice
Methylcholanthrene
Neoplasms
tissue culture
N-methyl-N-nitrosourea
thymoma
Methylnitrosourea
Thymoma
Viral Proteins
carcinogens
Carcinogens
genomics
Messenger RNA
DNA

ASJC Scopus subject areas

  • Immunology

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Absence of mouse mammary tumor virus proviral amplification in chemically induced lymphomas of RF/J mice. / Kraft, R.; Ishizaka, S. T.; Okenquist, S. A.; Childs, Geoffrey J.; Lilly, F.; Lenz, Jack.

In: Journal of Virology, Vol. 63, No. 7, 1989, p. 3200-3204.

Research output: Contribution to journalArticle

Kraft, R. ; Ishizaka, S. T. ; Okenquist, S. A. ; Childs, Geoffrey J. ; Lilly, F. ; Lenz, Jack. / Absence of mouse mammary tumor virus proviral amplification in chemically induced lymphomas of RF/J mice. In: Journal of Virology. 1989 ; Vol. 63, No. 7. pp. 3200-3204.
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