Abnormal chloride conductance in multidrug resistant HL60 AR cells

S. Gollapudi; T. McDonald; P. Gardner; N. Kang; S. Gupta

doi:10.1016/0304-3835(92)90284-3

Abnormal chloride conductance in multidrug resistant HL60 AR cells

S. Gollapudi, T. McDonald, P. Gardner, N. Kang, S. Gupta

Research output: Contribution to journal › Article › peer-review

15 Scopus citations

Abstract

Chloride channel currents were measured in drug sensitive parental HL60 and multidrug resistant (MDR) subline HL60 AR cells, using a whole cell patch-clamp technique. In addition, the in vitro effects of 4,4′ diisothiocyana-tostilbene-2,2′-disulfonic acid (DIDS), a Cl^- channel blocker, on intracellular accumulation and sensitivity to daunorubicin and intracellular pH (pH_i) in HL60 cells were examined. Baseline DIDS blockable Cl^- currents were consistently lower in HL60 AR cells (0.9 pA/pF) as compared to HL60 cells (7.0 pA/pF). Similarly cAMP-activated Cl^- currents were minimal in HL60 AR cells (0.2 pA/pF) as compared to HL60 cells (8 pA/pF). In vitro treatment of drug sensitive HL60 cells with DIDS resulted in concentration-dependent decreased accumulation and increased resistance to daunorubicin and decreased pH_i. These data show that altered Cl^- permeability is associated with MDR and suggest that Cl^- channels may play a role in MDR.

Original language	English (US)
Pages (from-to)	83-89
Number of pages	7
Journal	Cancer Letters
Volume	66
Issue number	1
DOIs	https://doi.org/10.1016/0304-3835(92)90284-3
State	Published - Sep 14 1992
Externally published	Yes

Keywords

chloride current
intracellular pH
multidrug resistance

ASJC Scopus subject areas

Oncology
Cancer Research

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/0304-3835(92)90284-3

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title = "Abnormal chloride conductance in multidrug resistant HL60 AR cells",

abstract = "Chloride channel currents were measured in drug sensitive parental HL60 and multidrug resistant (MDR) subline HL60 AR cells, using a whole cell patch-clamp technique. In addition, the in vitro effects of 4,4′ diisothiocyana-tostilbene-2,2′-disulfonic acid (DIDS), a Cl- channel blocker, on intracellular accumulation and sensitivity to daunorubicin and intracellular pH (pHi) in HL60 cells were examined. Baseline DIDS blockable Cl- currents were consistently lower in HL60 AR cells (0.9 pA/pF) as compared to HL60 cells (7.0 pA/pF). Similarly cAMP-activated Cl- currents were minimal in HL60 AR cells (0.2 pA/pF) as compared to HL60 cells (8 pA/pF). In vitro treatment of drug sensitive HL60 cells with DIDS resulted in concentration-dependent decreased accumulation and increased resistance to daunorubicin and decreased pHi. These data show that altered Cl- permeability is associated with MDR and suggest that Cl- channels may play a role in MDR.",

keywords = "chloride current, intracellular pH, multidrug resistance",

author = "S. Gollapudi and T. McDonald and P. Gardner and N. Kang and S. Gupta",

note = "Funding Information: This work was supported in part by grants from USPHS Al 26 456 (SG) and DK 41324 (PG.P . Gardner is a recipient of the Borrough Wellcome Faculty Scholar Award in Clinical Pharmacology. T. McDonald is supported by NIH grant KllHL 02 397.",

year = "1992",

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doi = "10.1016/0304-3835(92)90284-3",

language = "English (US)",

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pages = "83--89",

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T1 - Abnormal chloride conductance in multidrug resistant HL60 AR cells

AU - Gollapudi, S.

AU - McDonald, T.

AU - Gardner, P.

AU - Kang, N.

AU - Gupta, S.

N1 - Funding Information: This work was supported in part by grants from USPHS Al 26 456 (SG) and DK 41324 (PG.P . Gardner is a recipient of the Borrough Wellcome Faculty Scholar Award in Clinical Pharmacology. T. McDonald is supported by NIH grant KllHL 02 397.

PY - 1992/9/14

Y1 - 1992/9/14

N2 - Chloride channel currents were measured in drug sensitive parental HL60 and multidrug resistant (MDR) subline HL60 AR cells, using a whole cell patch-clamp technique. In addition, the in vitro effects of 4,4′ diisothiocyana-tostilbene-2,2′-disulfonic acid (DIDS), a Cl- channel blocker, on intracellular accumulation and sensitivity to daunorubicin and intracellular pH (pHi) in HL60 cells were examined. Baseline DIDS blockable Cl- currents were consistently lower in HL60 AR cells (0.9 pA/pF) as compared to HL60 cells (7.0 pA/pF). Similarly cAMP-activated Cl- currents were minimal in HL60 AR cells (0.2 pA/pF) as compared to HL60 cells (8 pA/pF). In vitro treatment of drug sensitive HL60 cells with DIDS resulted in concentration-dependent decreased accumulation and increased resistance to daunorubicin and decreased pHi. These data show that altered Cl- permeability is associated with MDR and suggest that Cl- channels may play a role in MDR.

AB - Chloride channel currents were measured in drug sensitive parental HL60 and multidrug resistant (MDR) subline HL60 AR cells, using a whole cell patch-clamp technique. In addition, the in vitro effects of 4,4′ diisothiocyana-tostilbene-2,2′-disulfonic acid (DIDS), a Cl- channel blocker, on intracellular accumulation and sensitivity to daunorubicin and intracellular pH (pHi) in HL60 cells were examined. Baseline DIDS blockable Cl- currents were consistently lower in HL60 AR cells (0.9 pA/pF) as compared to HL60 cells (7.0 pA/pF). Similarly cAMP-activated Cl- currents were minimal in HL60 AR cells (0.2 pA/pF) as compared to HL60 cells (8 pA/pF). In vitro treatment of drug sensitive HL60 cells with DIDS resulted in concentration-dependent decreased accumulation and increased resistance to daunorubicin and decreased pHi. These data show that altered Cl- permeability is associated with MDR and suggest that Cl- channels may play a role in MDR.

KW - chloride current

KW - intracellular pH

KW - multidrug resistance

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Abnormal chloride conductance in multidrug resistant HL60 AR cells

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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