Abnormal chloride conductance in multidrug resistant HL60 AR cells

S. Gollapudi, T. McDonald, P. Gardner, N. Kang, S. Gupta

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

Chloride channel currents were measured in drug sensitive parental HL60 and multidrug resistant (MDR) subline HL60 AR cells, using a whole cell patch-clamp technique. In addition, the in vitro effects of 4,4′ diisothiocyana-tostilbene-2,2′-disulfonic acid (DIDS), a Cl- channel blocker, on intracellular accumulation and sensitivity to daunorubicin and intracellular pH (pHi) in HL60 cells were examined. Baseline DIDS blockable Cl- currents were consistently lower in HL60 AR cells (0.9 pA/pF) as compared to HL60 cells (7.0 pA/pF). Similarly cAMP-activated Cl- currents were minimal in HL60 AR cells (0.2 pA/pF) as compared to HL60 cells (8 pA/pF). In vitro treatment of drug sensitive HL60 cells with DIDS resulted in concentration-dependent decreased accumulation and increased resistance to daunorubicin and decreased pHi. These data show that altered Cl- permeability is associated with MDR and suggest that Cl- channels may play a role in MDR.

Original languageEnglish (US)
Pages (from-to)83-89
Number of pages7
JournalCancer Letters
Volume66
Issue number1
DOIs
StatePublished - Sep 14 1992

Keywords

  • chloride current
  • intracellular pH
  • multidrug resistance

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Fingerprint Dive into the research topics of 'Abnormal chloride conductance in multidrug resistant HL60 AR cells'. Together they form a unique fingerprint.

  • Cite this

    Gollapudi, S., McDonald, T., Gardner, P., Kang, N., & Gupta, S. (1992). Abnormal chloride conductance in multidrug resistant HL60 AR cells. Cancer Letters, 66(1), 83-89. https://doi.org/10.1016/0304-3835(92)90284-3