Ablation spectra of the human cornea

D. Cohen, Roy S. Chuck, G. Bearman, P. McDonnell, W. Grundfest

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Ablation of human corneal tissue with 193 nm excimer laser energy generates fluorescence in the near ultraviolet and visible regions of the spectrum. The fluorescence spectra from five human corneas were collected during ablation in vitro. We find that the fluorescence spectrum changes continuously as the cornea is ablated from the epithelial surface towards the endothelium. We reduced the dimensionality of the large data set resulting from each cornea by a principal components analysis. The three most significant principal component eigenvectors suffice to describe the observed spectral evolution, and independent analysis of each tissue sample produces a similar set of eigenvectors. The evolution of the calculated eigenvector weighting factors during ablation then corresponds to the observed spectral evolution. In fact, this evolution is qualitatively consistent between corneas. We suggest that this spectral evolution offers promise as a real-time surgical feedback tool.

Original languageEnglish (US)
Pages (from-to)339-343
Number of pages5
JournalJournal of Biomedical Optics
Volume6
Issue number3
DOIs
StatePublished - Jul 2001
Externally publishedYes

Fingerprint

cornea
Ablation
Eigenvalues and eigenfunctions
Cornea
ablation
Fluorescence
eigenvectors
Tissue
fluorescence
Excimer lasers
Excimer Lasers
Principal component analysis
endothelium
Principal Component Analysis
Endothelium
Feedback
principal components analysis
excimer lasers

Keywords

  • Ablation
  • Cornea
  • Fluorescence
  • Lasers

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Radiology Nuclear Medicine and imaging
  • Atomic and Molecular Physics, and Optics
  • Radiological and Ultrasound Technology

Cite this

Cohen, D., Chuck, R. S., Bearman, G., McDonnell, P., & Grundfest, W. (2001). Ablation spectra of the human cornea. Journal of Biomedical Optics, 6(3), 339-343. https://doi.org/10.1117/1.1380670

Ablation spectra of the human cornea. / Cohen, D.; Chuck, Roy S.; Bearman, G.; McDonnell, P.; Grundfest, W.

In: Journal of Biomedical Optics, Vol. 6, No. 3, 07.2001, p. 339-343.

Research output: Contribution to journalArticle

Cohen, D, Chuck, RS, Bearman, G, McDonnell, P & Grundfest, W 2001, 'Ablation spectra of the human cornea', Journal of Biomedical Optics, vol. 6, no. 3, pp. 339-343. https://doi.org/10.1117/1.1380670
Cohen D, Chuck RS, Bearman G, McDonnell P, Grundfest W. Ablation spectra of the human cornea. Journal of Biomedical Optics. 2001 Jul;6(3):339-343. https://doi.org/10.1117/1.1380670
Cohen, D. ; Chuck, Roy S. ; Bearman, G. ; McDonnell, P. ; Grundfest, W. / Ablation spectra of the human cornea. In: Journal of Biomedical Optics. 2001 ; Vol. 6, No. 3. pp. 339-343.
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