A vapbc toxin-antitoxin module is a posttranscriptional regulator of metabolic flux in mycobacteria

Joanna L. McKenzie, Jennifer Robson, Michael Berney, Tony C. Smith, Alaine Ruthe, Paul P. Gardner, Vickery L. Arcus, Gregory M. Cook

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

The largest family of toxin-antitoxin (TA) modules are encoded by the vapBC operons, but their roles in bacterial physiology remain enigmatic. Microarray analysis in Mycobacterium smegmatis overexpressing VapC/VapBC revealed a high percentage of downregulated genes with annotated roles in carbon transport and metabolism, suggesting that VapC was targeting specific metabolic mRNA transcripts. To validate this hypothesis, purified VapC was used to identify the RNA cleavage site in vitro. VapC had RNase activity that was sequence specific, cleaving single-stranded RNA substrates at AUAU and AUAA in vitro and in vivo (viz., MSMEG_2121 to MSMEG_2124). A bioinformatic analysis of these regions suggested that an RNA hairpin 3= of the AUA(U/A) motif is also required for efficient cleavage. VapC-mediated regulation in vivo was demonstrated by showing that MSMEG_2124 (dhaF) and MSMEG_2121 (dhaM) were upregulated in a ΔvapBC mutant growing on glycerol. The ΔvapBC mutant had a specific rate of glycerol consumption that was 2.4-fold higher than that of the wild type during exponential growth. This increased rate of glycerol consumption was not used for generating bacterial biomass, suggesting that metabolism by the ΔvapBC mutant was uncoupled from growth. These data suggest a model in which VapC regulates the rate of glycerol utilization to match the anabolic demands of the cell, allowing for fine-tuning of the catabolic rate at a posttranscriptional level.

Original languageEnglish (US)
Pages (from-to)2189-2204
Number of pages16
JournalJournal of Bacteriology
Volume194
Issue number9
DOIs
StatePublished - May 2012
Externally publishedYes

Fingerprint

Antitoxins
Mycobacterium
Glycerol
Bacterial Physiological Phenomena
RNA Cleavage
RNA
Mycobacterium smegmatis
Microarray Analysis
Operon
Ribonucleases
Growth
Computational Biology
Biomass
Carbon
Down-Regulation
Messenger RNA
Genes
In Vitro Techniques

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

Cite this

McKenzie, J. L., Robson, J., Berney, M., Smith, T. C., Ruthe, A., Gardner, P. P., ... Cook, G. M. (2012). A vapbc toxin-antitoxin module is a posttranscriptional regulator of metabolic flux in mycobacteria. Journal of Bacteriology, 194(9), 2189-2204. https://doi.org/10.1128/JB.06790-11

A vapbc toxin-antitoxin module is a posttranscriptional regulator of metabolic flux in mycobacteria. / McKenzie, Joanna L.; Robson, Jennifer; Berney, Michael; Smith, Tony C.; Ruthe, Alaine; Gardner, Paul P.; Arcus, Vickery L.; Cook, Gregory M.

In: Journal of Bacteriology, Vol. 194, No. 9, 05.2012, p. 2189-2204.

Research output: Contribution to journalArticle

McKenzie, JL, Robson, J, Berney, M, Smith, TC, Ruthe, A, Gardner, PP, Arcus, VL & Cook, GM 2012, 'A vapbc toxin-antitoxin module is a posttranscriptional regulator of metabolic flux in mycobacteria', Journal of Bacteriology, vol. 194, no. 9, pp. 2189-2204. https://doi.org/10.1128/JB.06790-11
McKenzie, Joanna L. ; Robson, Jennifer ; Berney, Michael ; Smith, Tony C. ; Ruthe, Alaine ; Gardner, Paul P. ; Arcus, Vickery L. ; Cook, Gregory M. / A vapbc toxin-antitoxin module is a posttranscriptional regulator of metabolic flux in mycobacteria. In: Journal of Bacteriology. 2012 ; Vol. 194, No. 9. pp. 2189-2204.
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