A V region mutant producing an antibody that had lost the ability to bind phosphocholine was isolated from a hybridoma producing a germline encoded T15 antibody. The mutation resulted in a single aspartic acid to asparagine substitution at residue 95 of the H chain V region. This confirms that the aspartic acid at residue 95 plays a major role in Ag binding. The results also suggest that somatic cell genetic techniques can be used to generate mAb with useful changes in Ag binding.
|Original language||English (US)|
|Number of pages||4|
|Journal||Journal of Immunology|
|State||Published - Jan 1 1991|
ASJC Scopus subject areas
- Immunology and Allergy