A-type voltage-gated K+ currents influence firing properties of isolectin B4-positive but not isolectin B4-negative primary sensory neurons

Amaresh Vydyanathan, Zi Zhen Wu, Shao Rui Chen, Hui Lin Pan

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Abstract

Voltage-gated K+ channels (Kv) in primary sensory neurons are important for regulation of neuronal excitability. The dorsal root ganglion (DRG) neurons are heterogeneous, and the types of native Kv currents in different groups of nociceptive DRG neurons are not fully known. In this study, we determined the difference in the A-type Kv current and its influence on the firing properties between isolectin B4 (IB4)-positive and -negative DRG neurons. Whole cell voltage- and current-clamp recordings were performed on acutely dissociated small DRG neurons of rats. The total Kv current density was significantly higher in IB4-positive than that in IB4-negative neurons. Also, 4-aminopyridine (4-AP) produced a significantly greater reduction in Kv currents in IB4-positive than in IB4-negative neurons. In contrast, IB4-negative neurons exhibited a larger proportion of tetraethylammonium-sensitive Kv currents. Furthermore, IB4-positive neurons showed a longer latency of firing and required a significantly larger amount of current injection to evoke action potentials. 4-AP significantly decreased the latency of firing and increased the firing frequency in IB4-positive but not in IB4-negative neurons. Additionally, IB4-positive neurons are immunoreactive to Kv1.4 but not to Kv1.1 and Kv1.2 subunits. Collectively, this study provides new information that 4-AP-sensitive A-type Kv currents are mainly present in IB4-positive DRG neurons and preferentially dampen the initiation of action potentials of this subpopulation of nociceptors. The difference in the density of A-type Kv currents contributes to the distinct electrophysiological properties of IB4-positive and -negative DRG neurons.

Original languageEnglish (US)
Pages (from-to)3401-3409
Number of pages9
JournalJournal of Neurophysiology
Volume93
Issue number6
DOIs
StatePublished - Jun 2005
Externally publishedYes

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Sensory Receptor Cells
Lectins
Neurons
Spinal Ganglia
4-Aminopyridine
Action Potentials
Voltage-Gated Potassium Channels
Nociceptors
Tetraethylammonium

ASJC Scopus subject areas

  • Physiology
  • Neuroscience(all)

Cite this

A-type voltage-gated K+ currents influence firing properties of isolectin B4-positive but not isolectin B4-negative primary sensory neurons. / Vydyanathan, Amaresh; Wu, Zi Zhen; Chen, Shao Rui; Pan, Hui Lin.

In: Journal of Neurophysiology, Vol. 93, No. 6, 06.2005, p. 3401-3409.

Research output: Contribution to journalArticle

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