TY - JOUR
T1 - A transposon-related palindromic repetitive sequence from C. elegans
AU - Dreyfus, David H.
AU - Emmons, Scott W.
N1 - Funding Information:
We thank Drs. S. Baird, K.-L. Chow, and D. Fitch for tfieir comments on die manuscript. This work was supported by National Institutes of Health Grant GM38174 to SE. DHD is supported by National Institutes of Health grant T32 GM07288. SE is the recipient of die Harry Winston Established Fellowship for Research of die American Heart Association. Some nematode strains used in this study were provided by the Caenorhaditis Genetics Center, which is funded by die NTH National Center for Research Resources (NCRR).
PY - 1991/4/25
Y1 - 1991/4/25
N2 - A family of transposon-like sequences in the C. elegans genome is described. This family, termed the Tc6 family, consists mostly of conserved, 1.6 kb elements. Four Tc6 elements or partial elements have been cloned and the DNA sequences of three were determined. One appears to be a complete element of 1603 nucleotides, consisting of a palindrome of 765 nucleotides, with a central, non-palindromic region of 73 nucleotides. Another has an identical structure except for an intemal deletion. A third is a partial element terminating at a probable internal restriction site used for cloning. A fourth clone contained portions of the Tc6 sequence juxtaposed to non-Tc6 sequences. All C. elegans strains examined contain 20-30 Tc6 elements. The ends of Tc6 elements are conserved and have sequence similarity to the ends of C. elegans transposons Tc1 and Tc3. The ends of Tc6 elements also have sequence similarity to the heptamer portion of the immunogiobuiin and T-cell receptor recombination signal sequence, raising the possibility of wide phylogenetic conservation of the recombination mechanism. Tc6 elements also share sequence motifs with plant-pathogenic viroid RNA's, possibly indicative of a Tc6 RNA replicative phase.
AB - A family of transposon-like sequences in the C. elegans genome is described. This family, termed the Tc6 family, consists mostly of conserved, 1.6 kb elements. Four Tc6 elements or partial elements have been cloned and the DNA sequences of three were determined. One appears to be a complete element of 1603 nucleotides, consisting of a palindrome of 765 nucleotides, with a central, non-palindromic region of 73 nucleotides. Another has an identical structure except for an intemal deletion. A third is a partial element terminating at a probable internal restriction site used for cloning. A fourth clone contained portions of the Tc6 sequence juxtaposed to non-Tc6 sequences. All C. elegans strains examined contain 20-30 Tc6 elements. The ends of Tc6 elements are conserved and have sequence similarity to the ends of C. elegans transposons Tc1 and Tc3. The ends of Tc6 elements also have sequence similarity to the heptamer portion of the immunogiobuiin and T-cell receptor recombination signal sequence, raising the possibility of wide phylogenetic conservation of the recombination mechanism. Tc6 elements also share sequence motifs with plant-pathogenic viroid RNA's, possibly indicative of a Tc6 RNA replicative phase.
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U2 - 10.1093/nar/19.8.1871
DO - 10.1093/nar/19.8.1871
M3 - Article
C2 - 1674369
AN - SCOPUS:0025732383
SN - 0305-1048
VL - 19
SP - 1871
EP - 1878
JO - Nucleic acids research
JF - Nucleic acids research
IS - 8
ER -