A transgenic mouse for in vivo detection of endogenous labeled mRNA

Timothée Lionnet, Kevin Czaplinski, Xavier Darzacq, Yaron Shav-Tal, Amber L. Wells, Jeffrey A. Chao, Hye Yoon Park, Valeria De Turris, Melissa Lopez-Jones, Robert H. Singer

Research output: Contribution to journalArticle

191 Citations (Scopus)

Abstract

Live-cell single mRNA imaging is a powerful tool but has been restricted in higher eukaryotes to artificial cell lines and reporter genes. We describe an approach that enables live-cell imaging of single endogenous labeled mRNA molecules transcribed in primary mammalian cells and tissue. We generated a knock-in mouse line with an MS2 binding site (MBS) cassette targeted to the 3-2 untranslated region of the essential β-actin gene. As β-actin-MBS was ubiquitously expressed, we could uniquely address endogenous mRNA regulation in any tissue or cell type. We simultaneously followed transcription from the β-actin alleles in real time and observed transcriptional bursting in response to serum stimulation with precise temporal resolution. We tracked single endogenous labeled mRNA particles being transported in primary hippocampal neurons. The MBS cassette also enabled high-sensitivity fluorescence in situ hybridization (FISH), allowing detection and localization of single β-actin mRNA molecules in various mouse tissues.

Original languageEnglish (US)
Pages (from-to)165-170
Number of pages6
JournalNature Methods
Volume8
Issue number2
DOIs
StatePublished - Feb 2011

Fingerprint

Transgenic Mice
Actins
Messenger RNA
Binding Sites
Tissue
Genes
Cells
Artificial Cells
Untranslated Regions
Imaging techniques
Molecules
Essential Genes
3' Untranslated Regions
Transcription
Eukaryota
Fluorescence In Situ Hybridization
Reporter Genes
Neurons
Fluorescence
Alleles

ASJC Scopus subject areas

  • Biotechnology
  • Molecular Biology
  • Biochemistry
  • Cell Biology

Cite this

Lionnet, T., Czaplinski, K., Darzacq, X., Shav-Tal, Y., Wells, A. L., Chao, J. A., ... Singer, R. H. (2011). A transgenic mouse for in vivo detection of endogenous labeled mRNA. Nature Methods, 8(2), 165-170. https://doi.org/10.1038/nmeth.1551

A transgenic mouse for in vivo detection of endogenous labeled mRNA. / Lionnet, Timothée; Czaplinski, Kevin; Darzacq, Xavier; Shav-Tal, Yaron; Wells, Amber L.; Chao, Jeffrey A.; Park, Hye Yoon; De Turris, Valeria; Lopez-Jones, Melissa; Singer, Robert H.

In: Nature Methods, Vol. 8, No. 2, 02.2011, p. 165-170.

Research output: Contribution to journalArticle

Lionnet, T, Czaplinski, K, Darzacq, X, Shav-Tal, Y, Wells, AL, Chao, JA, Park, HY, De Turris, V, Lopez-Jones, M & Singer, RH 2011, 'A transgenic mouse for in vivo detection of endogenous labeled mRNA', Nature Methods, vol. 8, no. 2, pp. 165-170. https://doi.org/10.1038/nmeth.1551
Lionnet T, Czaplinski K, Darzacq X, Shav-Tal Y, Wells AL, Chao JA et al. A transgenic mouse for in vivo detection of endogenous labeled mRNA. Nature Methods. 2011 Feb;8(2):165-170. https://doi.org/10.1038/nmeth.1551
Lionnet, Timothée ; Czaplinski, Kevin ; Darzacq, Xavier ; Shav-Tal, Yaron ; Wells, Amber L. ; Chao, Jeffrey A. ; Park, Hye Yoon ; De Turris, Valeria ; Lopez-Jones, Melissa ; Singer, Robert H. / A transgenic mouse for in vivo detection of endogenous labeled mRNA. In: Nature Methods. 2011 ; Vol. 8, No. 2. pp. 165-170.
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