A toll-like receptor 9 (rs352140) variant is associated with placental inflammation in newborn infants

Vijender Karody, Shawn Reese, Navin Kumar, Jennifer L. Liedel, Jason Jarzembowski, Venkatesh Sampath

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Objective: Chorioamnionitis contributes to premature birth and associated postnatal morbidity. The genetic basis of altered immune responses underlying placental inflammation (PI) remains understudied. The aim of this study was to evaluate the relationship among TLR signaling pathway polymorphisms and different patterns of PI.Methods: Prospective cohort study in infants involving cord blood collection and placental examination for PI. One hundred and fifty-nine infants enrolled in study out of which 28 were term (eight with PI) and 131 preterm (47 with PI). DNA from blood was genotyped for SNPs in TLR2, 4, 5, 9, NFKBI, NFKBIA, TIRAP, and IRAK1 genes using multiplexed single base extension assay.Results: While there were no differences in BW, GA, gender, race, and SPL among infants with or without PI, there was a higher incidence of PPROM, maternal smoking, drug use, and clinical chorioamnionitis among infants with PI. Out of nine TLR variants, only CT and/or TT genotypes of the TLR9 variant (rs352140) were significantly associated (p = 0.004) with any PI and maternal pattern of inflammation (p = 0.012) both by univariate analysis and logistic regression.Conclusions: The presence of a variant T allele in a common SNP (rs352140) in the TLR9 gene whose product recognizes bacterial DNA is associated with increased PI.

Original languageEnglish (US)
Pages (from-to)2210-2216
Number of pages7
JournalJournal of Maternal-Fetal and Neonatal Medicine
Volume29
Issue number13
DOIs
StatePublished - Jul 2 2016

Fingerprint

Toll-Like Receptor 9
Newborn Infant
Inflammation
Chorioamnionitis
Single Nucleotide Polymorphism
Mothers
Bacterial DNA
Premature Birth
Fetal Blood
Genes
Cohort Studies
Logistic Models
Smoking
Alleles
Genotype
Prospective Studies

Keywords

  • Chorioamnionitis
  • innate immunity
  • placental inflammation
  • rs352140
  • toll-like receptor 9 polymorphisms

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health
  • Obstetrics and Gynecology

Cite this

A toll-like receptor 9 (rs352140) variant is associated with placental inflammation in newborn infants. / Karody, Vijender; Reese, Shawn; Kumar, Navin; Liedel, Jennifer L.; Jarzembowski, Jason; Sampath, Venkatesh.

In: Journal of Maternal-Fetal and Neonatal Medicine, Vol. 29, No. 13, 02.07.2016, p. 2210-2216.

Research output: Contribution to journalArticle

Karody, Vijender ; Reese, Shawn ; Kumar, Navin ; Liedel, Jennifer L. ; Jarzembowski, Jason ; Sampath, Venkatesh. / A toll-like receptor 9 (rs352140) variant is associated with placental inflammation in newborn infants. In: Journal of Maternal-Fetal and Neonatal Medicine. 2016 ; Vol. 29, No. 13. pp. 2210-2216.
@article{cec9835baca5436f9f9e5eb6dacb3259,
title = "A toll-like receptor 9 (rs352140) variant is associated with placental inflammation in newborn infants",
abstract = "Objective: Chorioamnionitis contributes to premature birth and associated postnatal morbidity. The genetic basis of altered immune responses underlying placental inflammation (PI) remains understudied. The aim of this study was to evaluate the relationship among TLR signaling pathway polymorphisms and different patterns of PI.Methods: Prospective cohort study in infants involving cord blood collection and placental examination for PI. One hundred and fifty-nine infants enrolled in study out of which 28 were term (eight with PI) and 131 preterm (47 with PI). DNA from blood was genotyped for SNPs in TLR2, 4, 5, 9, NFKBI, NFKBIA, TIRAP, and IRAK1 genes using multiplexed single base extension assay.Results: While there were no differences in BW, GA, gender, race, and SPL among infants with or without PI, there was a higher incidence of PPROM, maternal smoking, drug use, and clinical chorioamnionitis among infants with PI. Out of nine TLR variants, only CT and/or TT genotypes of the TLR9 variant (rs352140) were significantly associated (p = 0.004) with any PI and maternal pattern of inflammation (p = 0.012) both by univariate analysis and logistic regression.Conclusions: The presence of a variant T allele in a common SNP (rs352140) in the TLR9 gene whose product recognizes bacterial DNA is associated with increased PI.",
keywords = "Chorioamnionitis, innate immunity, placental inflammation, rs352140, toll-like receptor 9 polymorphisms",
author = "Vijender Karody and Shawn Reese and Navin Kumar and Liedel, {Jennifer L.} and Jason Jarzembowski and Venkatesh Sampath",
year = "2016",
month = "7",
day = "2",
doi = "10.3109/14767058.2015.1081590",
language = "English (US)",
volume = "29",
pages = "2210--2216",
journal = "Journal of Maternal-Fetal and Neonatal Medicine",
issn = "1476-7058",
publisher = "Informa Healthcare",
number = "13",

}

TY - JOUR

T1 - A toll-like receptor 9 (rs352140) variant is associated with placental inflammation in newborn infants

AU - Karody, Vijender

AU - Reese, Shawn

AU - Kumar, Navin

AU - Liedel, Jennifer L.

AU - Jarzembowski, Jason

AU - Sampath, Venkatesh

PY - 2016/7/2

Y1 - 2016/7/2

N2 - Objective: Chorioamnionitis contributes to premature birth and associated postnatal morbidity. The genetic basis of altered immune responses underlying placental inflammation (PI) remains understudied. The aim of this study was to evaluate the relationship among TLR signaling pathway polymorphisms and different patterns of PI.Methods: Prospective cohort study in infants involving cord blood collection and placental examination for PI. One hundred and fifty-nine infants enrolled in study out of which 28 were term (eight with PI) and 131 preterm (47 with PI). DNA from blood was genotyped for SNPs in TLR2, 4, 5, 9, NFKBI, NFKBIA, TIRAP, and IRAK1 genes using multiplexed single base extension assay.Results: While there were no differences in BW, GA, gender, race, and SPL among infants with or without PI, there was a higher incidence of PPROM, maternal smoking, drug use, and clinical chorioamnionitis among infants with PI. Out of nine TLR variants, only CT and/or TT genotypes of the TLR9 variant (rs352140) were significantly associated (p = 0.004) with any PI and maternal pattern of inflammation (p = 0.012) both by univariate analysis and logistic regression.Conclusions: The presence of a variant T allele in a common SNP (rs352140) in the TLR9 gene whose product recognizes bacterial DNA is associated with increased PI.

AB - Objective: Chorioamnionitis contributes to premature birth and associated postnatal morbidity. The genetic basis of altered immune responses underlying placental inflammation (PI) remains understudied. The aim of this study was to evaluate the relationship among TLR signaling pathway polymorphisms and different patterns of PI.Methods: Prospective cohort study in infants involving cord blood collection and placental examination for PI. One hundred and fifty-nine infants enrolled in study out of which 28 were term (eight with PI) and 131 preterm (47 with PI). DNA from blood was genotyped for SNPs in TLR2, 4, 5, 9, NFKBI, NFKBIA, TIRAP, and IRAK1 genes using multiplexed single base extension assay.Results: While there were no differences in BW, GA, gender, race, and SPL among infants with or without PI, there was a higher incidence of PPROM, maternal smoking, drug use, and clinical chorioamnionitis among infants with PI. Out of nine TLR variants, only CT and/or TT genotypes of the TLR9 variant (rs352140) were significantly associated (p = 0.004) with any PI and maternal pattern of inflammation (p = 0.012) both by univariate analysis and logistic regression.Conclusions: The presence of a variant T allele in a common SNP (rs352140) in the TLR9 gene whose product recognizes bacterial DNA is associated with increased PI.

KW - Chorioamnionitis

KW - innate immunity

KW - placental inflammation

KW - rs352140

KW - toll-like receptor 9 polymorphisms

UR - http://www.scopus.com/inward/record.url?scp=84941662570&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84941662570&partnerID=8YFLogxK

U2 - 10.3109/14767058.2015.1081590

DO - 10.3109/14767058.2015.1081590

M3 - Article

VL - 29

SP - 2210

EP - 2216

JO - Journal of Maternal-Fetal and Neonatal Medicine

JF - Journal of Maternal-Fetal and Neonatal Medicine

SN - 1476-7058

IS - 13

ER -