A technique for transplantation of ovarian cortical strips to the forearm

Kutluk Oktay, Erkan Buyuk, Zev Rosenwaks, James Rucinski

Research output: Contribution to journalArticle

79 Citations (Scopus)

Abstract

Objective: To describe a forearm heterotopic ovarian transplantation technique. Design: Case study. Setting: Academic medical center. Patient(s): One patient with stage IIIB squamous cell cervical carcinoma and one patient with recurrent benign ovarian cysts. Intervention(s): Preparation of thin ovarian cortical slices and transplantation under the skin of the forearm. Main Outcome Measure(s): Follicular development and oocyte retrieval; cyclical estradiol (E2) and progesterone (P4) production; restoration of serum follicle-stimulating hormone (FSH) and luteinizing hormone (LH) levels to reproductive age range. Result(s): Both patients were menopausal immediately after oophorectomy. The first patient developed a dominant follicle 10 weeks after transplantation, and her gonadotropin levels decreased to nonmenopausal levels. Percutaneous aspiration of ovarian follicles yielded a metaphase I (M-I) oocyte that was matured to metaphase II (M-II). The first patient's graft was functional for at least 21 months. In the second patient, ovarian follicle development was detected 6 months after transplantation, and periodic menstruation occurred thereafter. Spontaneous ovulation was confirmed by a midluteal increase in her P4 levels. Menstruation and follicle development continued for more than 2 years after the transplant. Conclusion(s): Heterotopic transplantation of ovarian tissue to the forearm is a simple and promising technique to restore ovarian function in women who become menopausal due to chemotherapy, surgery, or radiation.

Original languageEnglish (US)
Pages (from-to)193-198
Number of pages6
JournalFertility and Sterility
Volume80
Issue number1
DOIs
StatePublished - Jul 1 2003
Externally publishedYes

Fingerprint

Forearm
Transplantation
Heterotopic Transplantation
Ovarian Follicle
Menstruation
Metaphase
Transplants
Oocyte Retrieval
Ovarian Cysts
Skin Transplantation
Follicle Stimulating Hormone
Ovariectomy
Luteinizing Hormone
Ovulation
Gonadotropins
Oocytes
Progesterone
Squamous Cell Carcinoma
Estradiol
Outcome Assessment (Health Care)

Keywords

  • Cancer
  • Fertility preservation
  • Forearm
  • Heterotopic
  • Ovarian transplant

ASJC Scopus subject areas

  • Obstetrics and Gynecology

Cite this

A technique for transplantation of ovarian cortical strips to the forearm. / Oktay, Kutluk; Buyuk, Erkan; Rosenwaks, Zev; Rucinski, James.

In: Fertility and Sterility, Vol. 80, No. 1, 01.07.2003, p. 193-198.

Research output: Contribution to journalArticle

Oktay, Kutluk ; Buyuk, Erkan ; Rosenwaks, Zev ; Rucinski, James. / A technique for transplantation of ovarian cortical strips to the forearm. In: Fertility and Sterility. 2003 ; Vol. 80, No. 1. pp. 193-198.
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