A Tbx1-Six1/Eya1-Fgf8 genetic pathway controls mammalian cardiovascular and craniofacial morphogenesis

Chaoshe Guo, Ye Sun, Bin Zhou, Rosalyn M. Adam, XiaoKun Li, William T. Pu, Bernice E. Morrow, Anne Moon, Xue Li

Research output: Contribution to journalArticle

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Abstract

Shared molecular programs govern the formation of heart and head during mammalian embryogenesis. Development of both structures is disrupted in human chromosomal microdeletion of 22q11.2 (del22q11), which causes DiGeorge syndrome (DGS) and velo-cardio-facial syndrome (VCFS). Here, we have identified a genetic pathway involving the Six1/Eya1 transcription complex that regulates cardiovascular and craniofacial development. We demonstrate that murine mutation of both Six1 and Eya1 recapitulated most features of human del22q11 syndromes, including craniofacial, cardiac outflow tract, and aortic arch malformations. The mutant phenotypes were attributable in part to a reduction of fibroblast growth factor 8 (Fgf8), which was shown to be a direct downstream effector of Six1 and Eya1. Furthermore, we showed that Six1 and Eya1 genetically interacted with Fgf8 and the critical del22q11 gene T-box transcription factor 1 (Tbx1) in mice. Together, these findings reveal a Tbx1-Six1/Eya1-Fgf8 genetic pathway that is crucial for mammalian cardiocraniofacial morphogenesis and provide insights into the pathogenesis of human del22q11 syndromes.

Original languageEnglish (US)
Pages (from-to)1585-1595
Number of pages11
JournalJournal of Clinical Investigation
Volume121
Issue number4
DOIs
StatePublished - Apr 1 2011

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Fibroblast Growth Factor 8
Morphogenesis
DiGeorge Syndrome
Thoracic Aorta
Embryonic Development
Transcription Factors
Head
Phenotype
Mutation
Genes

ASJC Scopus subject areas

  • Medicine(all)

Cite this

A Tbx1-Six1/Eya1-Fgf8 genetic pathway controls mammalian cardiovascular and craniofacial morphogenesis. / Guo, Chaoshe; Sun, Ye; Zhou, Bin; Adam, Rosalyn M.; Li, XiaoKun; Pu, William T.; Morrow, Bernice E.; Moon, Anne; Li, Xue.

In: Journal of Clinical Investigation, Vol. 121, No. 4, 01.04.2011, p. 1585-1595.

Research output: Contribution to journalArticle

Guo, Chaoshe ; Sun, Ye ; Zhou, Bin ; Adam, Rosalyn M. ; Li, XiaoKun ; Pu, William T. ; Morrow, Bernice E. ; Moon, Anne ; Li, Xue. / A Tbx1-Six1/Eya1-Fgf8 genetic pathway controls mammalian cardiovascular and craniofacial morphogenesis. In: Journal of Clinical Investigation. 2011 ; Vol. 121, No. 4. pp. 1585-1595.
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