A system of counteracting feedback loops regulates Cdc42p activity during spontaneous cell polarization

Ertugrul M. Ozbudak, Attila Becskei, Alexander van Oudenaarden

Research output: Contribution to journalArticle

89 Citations (Scopus)

Abstract

Cellular polarization is often a response to distinct extracellular or intracellular cues, such as nutrient gradients or cortical landmarks. However, in the absence of such cues, some cells can still select a polarization axis at random. Positive feedback loops promoting localized activation of the GTPase Cdc42p are central to this process in budding yeast. Here, we explore spontaneous polarization during bud site selection in mutant yeast cells that lack functional landmarks. We find that these cells do not select a single random polarization axis, but continuously change this axis during the G1 phase of the cell cycle. This is reflected in traveling waves of activated Cdc42p which randomly explore the cell periphery. Our integrated computational and in vivo analyses of these waves reveal a negative feedback loop that competes with the aforementioned positive feedback loops to regulate Cdc42p activity and confer dynamic responsiveness on the robust initiation of cell polarization.

Original languageEnglish (US)
Pages (from-to)565-571
Number of pages7
JournalDevelopmental Cell
Volume9
Issue number4
DOIs
StatePublished - Oct 2005
Externally publishedYes

Fingerprint

Polarization
Feedback
Yeast
Cues
Cells
Site selection
Saccharomycetales
GTP Phosphohydrolases
G1 Phase
Nutrients
Cell Cycle
Yeasts
Chemical activation
Food

ASJC Scopus subject areas

  • Developmental Biology

Cite this

A system of counteracting feedback loops regulates Cdc42p activity during spontaneous cell polarization. / Ozbudak, Ertugrul M.; Becskei, Attila; van Oudenaarden, Alexander.

In: Developmental Cell, Vol. 9, No. 4, 10.2005, p. 565-571.

Research output: Contribution to journalArticle

Ozbudak, Ertugrul M. ; Becskei, Attila ; van Oudenaarden, Alexander. / A system of counteracting feedback loops regulates Cdc42p activity during spontaneous cell polarization. In: Developmental Cell. 2005 ; Vol. 9, No. 4. pp. 565-571.
@article{ef938bf3c7ca4744ba8878427963a8db,
title = "A system of counteracting feedback loops regulates Cdc42p activity during spontaneous cell polarization",
abstract = "Cellular polarization is often a response to distinct extracellular or intracellular cues, such as nutrient gradients or cortical landmarks. However, in the absence of such cues, some cells can still select a polarization axis at random. Positive feedback loops promoting localized activation of the GTPase Cdc42p are central to this process in budding yeast. Here, we explore spontaneous polarization during bud site selection in mutant yeast cells that lack functional landmarks. We find that these cells do not select a single random polarization axis, but continuously change this axis during the G1 phase of the cell cycle. This is reflected in traveling waves of activated Cdc42p which randomly explore the cell periphery. Our integrated computational and in vivo analyses of these waves reveal a negative feedback loop that competes with the aforementioned positive feedback loops to regulate Cdc42p activity and confer dynamic responsiveness on the robust initiation of cell polarization.",
author = "Ozbudak, {Ertugrul M.} and Attila Becskei and {van Oudenaarden}, Alexander",
year = "2005",
month = "10",
doi = "10.1016/j.devcel.2005.08.014",
language = "English (US)",
volume = "9",
pages = "565--571",
journal = "Developmental Cell",
issn = "1534-5807",
publisher = "Cell Press",
number = "4",

}

TY - JOUR

T1 - A system of counteracting feedback loops regulates Cdc42p activity during spontaneous cell polarization

AU - Ozbudak, Ertugrul M.

AU - Becskei, Attila

AU - van Oudenaarden, Alexander

PY - 2005/10

Y1 - 2005/10

N2 - Cellular polarization is often a response to distinct extracellular or intracellular cues, such as nutrient gradients or cortical landmarks. However, in the absence of such cues, some cells can still select a polarization axis at random. Positive feedback loops promoting localized activation of the GTPase Cdc42p are central to this process in budding yeast. Here, we explore spontaneous polarization during bud site selection in mutant yeast cells that lack functional landmarks. We find that these cells do not select a single random polarization axis, but continuously change this axis during the G1 phase of the cell cycle. This is reflected in traveling waves of activated Cdc42p which randomly explore the cell periphery. Our integrated computational and in vivo analyses of these waves reveal a negative feedback loop that competes with the aforementioned positive feedback loops to regulate Cdc42p activity and confer dynamic responsiveness on the robust initiation of cell polarization.

AB - Cellular polarization is often a response to distinct extracellular or intracellular cues, such as nutrient gradients or cortical landmarks. However, in the absence of such cues, some cells can still select a polarization axis at random. Positive feedback loops promoting localized activation of the GTPase Cdc42p are central to this process in budding yeast. Here, we explore spontaneous polarization during bud site selection in mutant yeast cells that lack functional landmarks. We find that these cells do not select a single random polarization axis, but continuously change this axis during the G1 phase of the cell cycle. This is reflected in traveling waves of activated Cdc42p which randomly explore the cell periphery. Our integrated computational and in vivo analyses of these waves reveal a negative feedback loop that competes with the aforementioned positive feedback loops to regulate Cdc42p activity and confer dynamic responsiveness on the robust initiation of cell polarization.

UR - http://www.scopus.com/inward/record.url?scp=26444469935&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=26444469935&partnerID=8YFLogxK

U2 - 10.1016/j.devcel.2005.08.014

DO - 10.1016/j.devcel.2005.08.014

M3 - Article

C2 - 16198298

AN - SCOPUS:26444469935

VL - 9

SP - 565

EP - 571

JO - Developmental Cell

JF - Developmental Cell

SN - 1534-5807

IS - 4

ER -