A synthetic glycolipid with B cell mitogenic activity

David L. Rosenstreich, J. Asselineau, S. E A Mergenhagen

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

A synthetic glycolipid, N palmitoyl d glucosamine, (NPG) was found to be a potent B cell mitogen, that stimulated spleen cell from nu/nu as well as from normal mice. The proper dispersion of the water insoluble preparation is critical for the elicitation of this mitogenic effect. Limulus lysate clotting assay indicated that the NPG preparation either contains only 0.001% endotoxin contamination or that NPG itself is 10-5 times less active in this assay than purified endotoxic LPS. Since such low levels of endotoxin concentration are not mitogenic, it is concluded that synthetic N palmitoyl d glucosamine, when properly dispersed, is itself a B cell mitogen.

Original languageEnglish (US)
Pages (from-to)1404-1409
Number of pages6
JournalJournal of Experimental Medicine
Volume140
Issue number5
StatePublished - 1974
Externally publishedYes

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Glucosamine
Glycolipids
B-Lymphocytes
Mitogens
Endotoxins
Horseshoe Crabs
Spleen
Water

ASJC Scopus subject areas

  • Immunology

Cite this

Rosenstreich, D. L., Asselineau, J., & Mergenhagen, S. E. A. (1974). A synthetic glycolipid with B cell mitogenic activity. Journal of Experimental Medicine, 140(5), 1404-1409.

A synthetic glycolipid with B cell mitogenic activity. / Rosenstreich, David L.; Asselineau, J.; Mergenhagen, S. E A.

In: Journal of Experimental Medicine, Vol. 140, No. 5, 1974, p. 1404-1409.

Research output: Contribution to journalArticle

Rosenstreich, DL, Asselineau, J & Mergenhagen, SEA 1974, 'A synthetic glycolipid with B cell mitogenic activity', Journal of Experimental Medicine, vol. 140, no. 5, pp. 1404-1409.
Rosenstreich, David L. ; Asselineau, J. ; Mergenhagen, S. E A. / A synthetic glycolipid with B cell mitogenic activity. In: Journal of Experimental Medicine. 1974 ; Vol. 140, No. 5. pp. 1404-1409.
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