A soluble lipid·protein complex from bovine adrenal medulla chromaffin granules

R. A. Hogue-Angeletti, P. B. Sheetz

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

A unique soluble lipoprotein has been isolated from aqueous lysates of bovine adrenal medulla chromaffin granules by DEAE-cellulose chromatography and gel filtration. Chloroform/methanol extracts of this complex contain sphingomyelin, lecithin, and cholesterol. Gel filtration in aqueous media indicates an approximate molecular weight of 900,000 for the complex. Incubation with sodium dodecyl sulfate causes dissociation to a low molecular weight polypeptide; prolonged treatment with guanidine HCl does not promote dissociation at all. Amino acid analysis revealed a high content of hydrophobic amino acids. Analysis of the tryptic fingerprint indicates that a single type of polypeptide chain is present. The complex appears to contain approximately five copies of polypeptide per aggregate.

Original languageEnglish (US)
Pages (from-to)5613-5616
Number of pages4
JournalJournal of Biological Chemistry
Volume253
Issue number16
StatePublished - 1978
Externally publishedYes

Fingerprint

Chromaffin Granules
Adrenal Medulla
Peptides
Gel Chromatography
Molecular Weight
Gels
Molecular weight
Amino Acids
DEAE-Cellulose Chromatography
DEAE-Cellulose
Sphingomyelins
Lecithins
Guanidine
Dermatoglyphics
Chloroform
Chromatography
Complex Mixtures
Sodium Dodecyl Sulfate
Lipoproteins
Methanol

ASJC Scopus subject areas

  • Biochemistry

Cite this

A soluble lipid·protein complex from bovine adrenal medulla chromaffin granules. / Hogue-Angeletti, R. A.; Sheetz, P. B.

In: Journal of Biological Chemistry, Vol. 253, No. 16, 1978, p. 5613-5616.

Research output: Contribution to journalArticle

Hogue-Angeletti, RA & Sheetz, PB 1978, 'A soluble lipid·protein complex from bovine adrenal medulla chromaffin granules', Journal of Biological Chemistry, vol. 253, no. 16, pp. 5613-5616.
Hogue-Angeletti, R. A. ; Sheetz, P. B. / A soluble lipid·protein complex from bovine adrenal medulla chromaffin granules. In: Journal of Biological Chemistry. 1978 ; Vol. 253, No. 16. pp. 5613-5616.
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