TY - JOUR
T1 - A soluble gradient of the neuropeptide secretoneurin promotes the transendothelial migration of monocytes in vitro
AU - Kähler, Christian M.
AU - Kaufmann, Gerhard
AU - Hogue-Angeletti, Ruth
AU - Fischer-Colbrie, Reiner
AU - Dunzendorfer, Stefan
AU - Reinisch, Norbert
AU - Wiedermann, Christian J.
N1 - Funding Information:
The work was supported by the Austrian Science Fund FWF no. 09977 and by the Austrian National Bank. The authors wish to thank Univ. Prof. R. Fischer-Colbrie, MD and Univ. Prof. H. Winkler, MD from the Department of Pharmacology, Faculty of Medicine, University of Innsbruck, for fruitful discussions and generously providing specific secretoneurin reagents.
PY - 1999/1/15
Y1 - 1999/1/15
N2 - Secretoneurin, derived from the chromogranin secretogranin II, triggers the selective migration of human monocytes, eosinophils, fibroblasts, endothelial and smooth muscle cells. More recently, we located specific binding sites on the human monocytic cell line MonoMac-6. Differentiated U937 transendothelial diapedesis was evaluated using an in vitro model of the vascular wall and specific monoclonal antibodies against CD11/CD18 and the α-chains of the very late activation antigen (VLA)-4 were used to evaluate involved adhesion molecules. Results showed a significant migratory response to secretoneurin between 10-8 to 10-10 M. Migration was comparable to a maximal effect induced by the monocyte chemotactic agent N-formyl-Met-Leu-Phe (fMLP, 10-8 M). Rabbit anti-secretoneurin antibodies were able to block the neuropeptide effect but not of fMLP and a trypsinized secretoneurin preparation as well as the secretogranin II-fragment EL-17 were ineffective in eliciting migration. Transmigration of U937 across endothelial-layers toward secretoneurin is inhibited by antibodies to CD11/CD18 adhesion molecules. The novel neuropeptide secretoneurin may play a role in regulating migration of monocytes into the subendothelial space, supposing a role in inflammatory responses. Copyright (C) 1999 Elsevier Science B.V.
AB - Secretoneurin, derived from the chromogranin secretogranin II, triggers the selective migration of human monocytes, eosinophils, fibroblasts, endothelial and smooth muscle cells. More recently, we located specific binding sites on the human monocytic cell line MonoMac-6. Differentiated U937 transendothelial diapedesis was evaluated using an in vitro model of the vascular wall and specific monoclonal antibodies against CD11/CD18 and the α-chains of the very late activation antigen (VLA)-4 were used to evaluate involved adhesion molecules. Results showed a significant migratory response to secretoneurin between 10-8 to 10-10 M. Migration was comparable to a maximal effect induced by the monocyte chemotactic agent N-formyl-Met-Leu-Phe (fMLP, 10-8 M). Rabbit anti-secretoneurin antibodies were able to block the neuropeptide effect but not of fMLP and a trypsinized secretoneurin preparation as well as the secretogranin II-fragment EL-17 were ineffective in eliciting migration. Transmigration of U937 across endothelial-layers toward secretoneurin is inhibited by antibodies to CD11/CD18 adhesion molecules. The novel neuropeptide secretoneurin may play a role in regulating migration of monocytes into the subendothelial space, supposing a role in inflammatory responses. Copyright (C) 1999 Elsevier Science B.V.
KW - CD11/CD18
KW - Endothelial cell
KW - Transmigration
KW - U937
KW - VLA-4 (very late activation antigen)
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U2 - 10.1016/S0014-2999(98)00814-0
DO - 10.1016/S0014-2999(98)00814-0
M3 - Article
C2 - 9988125
AN - SCOPUS:0033555941
SN - 0014-2999
VL - 365
SP - 65
EP - 75
JO - European Journal of Pharmacology
JF - European Journal of Pharmacology
IS - 1
ER -