A sodium dodecyl sulfate micro-gel-electrophoresis technique suitable for routine laboratory analysis

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

A micro-gel technique has been developed that permits routine analysis of protein down to 10-9 g. Gel preparation, electrophoresis, staining, and destaining can be completed in less than 6 hr. Because these micro-gels are intermediate in size between traditional tube gels and micro-gels used with single cells (1), preparation and handling are simplified, and quantitative densitometry can be performed directly on the gels using standard equipment. The resolution and reproducibility of these micro-gels are comparable to those of the traditional tube gel. In addition, the use of sodium dodecyl sulfate (SDS) with this micro-gel system permits routine molecular weight determination of micro-samples.

Original languageEnglish (US)
Pages (from-to)195-207
Number of pages13
JournalAnalytical Biochemistry
Volume77
Issue number1
DOIs
StatePublished - 1977
Externally publishedYes

Fingerprint

Electrophoresis
Sodium Dodecyl Sulfate
Gels
Densitometry
Molecular Weight
Molecular weight
Staining and Labeling
Equipment and Supplies

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

@article{b0a5a1965ef14e21a331898f5de3537d,
title = "A sodium dodecyl sulfate micro-gel-electrophoresis technique suitable for routine laboratory analysis",
abstract = "A micro-gel technique has been developed that permits routine analysis of protein down to 10-9 g. Gel preparation, electrophoresis, staining, and destaining can be completed in less than 6 hr. Because these micro-gels are intermediate in size between traditional tube gels and micro-gels used with single cells (1), preparation and handling are simplified, and quantitative densitometry can be performed directly on the gels using standard equipment. The resolution and reproducibility of these micro-gels are comparable to those of the traditional tube gel. In addition, the use of sodium dodecyl sulfate (SDS) with this micro-gel system permits routine molecular weight determination of micro-samples.",
author = "Condeelis, {John S.}",
year = "1977",
doi = "10.1016/0003-2697(77)90305-0",
language = "English (US)",
volume = "77",
pages = "195--207",
journal = "Analytical Biochemistry",
issn = "0003-2697",
publisher = "Academic Press Inc.",
number = "1",

}

TY - JOUR

T1 - A sodium dodecyl sulfate micro-gel-electrophoresis technique suitable for routine laboratory analysis

AU - Condeelis, John S.

PY - 1977

Y1 - 1977

N2 - A micro-gel technique has been developed that permits routine analysis of protein down to 10-9 g. Gel preparation, electrophoresis, staining, and destaining can be completed in less than 6 hr. Because these micro-gels are intermediate in size between traditional tube gels and micro-gels used with single cells (1), preparation and handling are simplified, and quantitative densitometry can be performed directly on the gels using standard equipment. The resolution and reproducibility of these micro-gels are comparable to those of the traditional tube gel. In addition, the use of sodium dodecyl sulfate (SDS) with this micro-gel system permits routine molecular weight determination of micro-samples.

AB - A micro-gel technique has been developed that permits routine analysis of protein down to 10-9 g. Gel preparation, electrophoresis, staining, and destaining can be completed in less than 6 hr. Because these micro-gels are intermediate in size between traditional tube gels and micro-gels used with single cells (1), preparation and handling are simplified, and quantitative densitometry can be performed directly on the gels using standard equipment. The resolution and reproducibility of these micro-gels are comparable to those of the traditional tube gel. In addition, the use of sodium dodecyl sulfate (SDS) with this micro-gel system permits routine molecular weight determination of micro-samples.

UR - http://www.scopus.com/inward/record.url?scp=0017579854&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0017579854&partnerID=8YFLogxK

U2 - 10.1016/0003-2697(77)90305-0

DO - 10.1016/0003-2697(77)90305-0

M3 - Article

VL - 77

SP - 195

EP - 207

JO - Analytical Biochemistry

JF - Analytical Biochemistry

SN - 0003-2697

IS - 1

ER -