Abstract
A micro-gel technique has been developed that permits routine analysis of protein down to 10-9 g. Gel preparation, electrophoresis, staining, and destaining can be completed in less than 6 hr. Because these micro-gels are intermediate in size between traditional tube gels and micro-gels used with single cells (1), preparation and handling are simplified, and quantitative densitometry can be performed directly on the gels using standard equipment. The resolution and reproducibility of these micro-gels are comparable to those of the traditional tube gel. In addition, the use of sodium dodecyl sulfate (SDS) with this micro-gel system permits routine molecular weight determination of micro-samples.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 195-207 |
| Number of pages | 13 |
| Journal | Analytical Biochemistry |
| Volume | 77 |
| Issue number | 1 |
| DOIs | |
| State | Published - 1977 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology
Cite this
A sodium dodecyl sulfate micro-gel-electrophoresis technique suitable for routine laboratory analysis. / Condeelis, John S.
In: Analytical Biochemistry, Vol. 77, No. 1, 1977, p. 195-207.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - A sodium dodecyl sulfate micro-gel-electrophoresis technique suitable for routine laboratory analysis
AU - Condeelis, John S.
PY - 1977
Y1 - 1977
N2 - A micro-gel technique has been developed that permits routine analysis of protein down to 10-9 g. Gel preparation, electrophoresis, staining, and destaining can be completed in less than 6 hr. Because these micro-gels are intermediate in size between traditional tube gels and micro-gels used with single cells (1), preparation and handling are simplified, and quantitative densitometry can be performed directly on the gels using standard equipment. The resolution and reproducibility of these micro-gels are comparable to those of the traditional tube gel. In addition, the use of sodium dodecyl sulfate (SDS) with this micro-gel system permits routine molecular weight determination of micro-samples.
AB - A micro-gel technique has been developed that permits routine analysis of protein down to 10-9 g. Gel preparation, electrophoresis, staining, and destaining can be completed in less than 6 hr. Because these micro-gels are intermediate in size between traditional tube gels and micro-gels used with single cells (1), preparation and handling are simplified, and quantitative densitometry can be performed directly on the gels using standard equipment. The resolution and reproducibility of these micro-gels are comparable to those of the traditional tube gel. In addition, the use of sodium dodecyl sulfate (SDS) with this micro-gel system permits routine molecular weight determination of micro-samples.
UR - http://www.scopus.com/inward/record.url?scp=0017579854&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0017579854&partnerID=8YFLogxK
U2 - 10.1016/0003-2697(77)90305-0
DO - 10.1016/0003-2697(77)90305-0
M3 - Article
VL - 77
SP - 195
EP - 207
JO - Analytical Biochemistry
JF - Analytical Biochemistry
SN - 0003-2697
IS - 1
ER -