TY - JOUR
T1 - A sodium dodecyl sulfate micro-gel-electrophoresis technique suitable for routine laboratory analysis
AU - Condeelis, J. S.
N1 - Funding Information:
This study was supported by Research Grants GM18854 (SUNY-Albany) and GM22356 (Dartmouth College) to Dr. R. D. Allen from the National Institute of General Medical Sciences.
PY - 1977/1
Y1 - 1977/1
N2 - A micro-gel technique has been developed that permits routine analysis of protein down to 10-9 g. Gel preparation, electrophoresis, staining, and destaining can be completed in less than 6 hr. Because these micro-gels are intermediate in size between traditional tube gels and micro-gels used with single cells (1), preparation and handling are simplified, and quantitative densitometry can be performed directly on the gels using standard equipment. The resolution and reproducibility of these micro-gels are comparable to those of the traditional tube gel. In addition, the use of sodium dodecyl sulfate (SDS) with this micro-gel system permits routine molecular weight determination of micro-samples.
AB - A micro-gel technique has been developed that permits routine analysis of protein down to 10-9 g. Gel preparation, electrophoresis, staining, and destaining can be completed in less than 6 hr. Because these micro-gels are intermediate in size between traditional tube gels and micro-gels used with single cells (1), preparation and handling are simplified, and quantitative densitometry can be performed directly on the gels using standard equipment. The resolution and reproducibility of these micro-gels are comparable to those of the traditional tube gel. In addition, the use of sodium dodecyl sulfate (SDS) with this micro-gel system permits routine molecular weight determination of micro-samples.
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U2 - 10.1016/0003-2697(77)90305-0
DO - 10.1016/0003-2697(77)90305-0
M3 - Article
C2 - 831571
AN - SCOPUS:0017579854
SN - 0003-2697
VL - 77
SP - 195
EP - 207
JO - Analytical Biochemistry
JF - Analytical Biochemistry
IS - 1
ER -