A small nucleolar RNA:ribozyme hybrid cleaves a nucleolar RNA target in vivo with near-perfect efficiency

Dmitry A. Samarsky, Gerardo Ferbeyre, Edouard Bertrand, Robert H. Singer, Robert Cedergren, Maurille J. Fournier

Research output: Contribution to journalArticle

46 Scopus citations

Abstract

A hammerhead ribozyme has been localized to the yeast nucleolus by using the U3 small nucleolar RNA as a carrier. The hybrid small nucleolar RNA:ribozyme, designated a 'snorbozyme,' is metabolically stable and cleaves a target U3 RNA with nearly 100% efficiency in vivo. This is the most efficient in vivo cleavage reported for a trans-acting ribozyme. A key advantage of the model substrate featured is that a stable, trimmed cleavage product accumulates. This property allows accurate kinetic measurements of authentic cleavage in vivo. The system offers new avenues for developing effective ribozymes for research and therapeutic applications.

Original languageEnglish (US)
Pages (from-to)6609-6614
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume96
Issue number12
DOIs
StatePublished - Jun 8 1999

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Keywords

  • Nucleolus
  • Yeast

ASJC Scopus subject areas

  • General

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