A single subset of dendritic cells controls the cytokine bias of natural killer T cell responses to diverse glycolipid antigens

Pooja Arora, Andres Baena, KarlO A. Yu, Neeraj K. Saini, Shalu Sharma, Michael F. Goldberg, Shajo Kunnath-Velayudhan, Leandro J. Carreño, Manjunatha M. Venkataswamy, John Kim, Eszter Lazar-Molnar, Gregoire Lauvau, Young tae Chang, Zheng Liu, Robert Bittman, Aymen Al-Shamkhani, Liam R. Cox, Peter J. Jervis, Natacha Veerapen, Gurdyal S. Besra & 1 others Steven A. Porcelli

Research output: Contribution to journalArticle

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Abstract

Many hematopoietic cell types express CD1d and are capable of presenting glycolipid antigens to invariant natural killer Tcells (iNKT cells). However, the question of which cells are the principal presenters of glycolipid antigens invivo remains controversial, and it has been suggested that this might vary depending on the structure of a particular glycolipid antigen. Here we have shown that a single type of cell, the CD8α+ DEC-205+ dendritic cell, was mainly responsible for capturing and presenting a variety of different glycolipid antigens, including multiple forms of α-galactosylceramide that stimulate widely divergent cytokine responses. After glycolipid presentation, these dendritic cells rapidly altered their expression of various costimulatory and coinhibitory molecules in a manner that was dependent on the structure of the antigen. These findings show flexibility in the outcome of two-way communication between CD8α+ dendritic cells and iNKT cells, providing a mechanism for biasing toward either proinflammatory or anti-inflammatory responses.

Original languageEnglish (US)
Pages (from-to)105-116
Number of pages12
JournalImmunity
Volume40
Issue number1
DOIs
StatePublished - Jan 16 2014

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Natural Killer T-Cells
Glycolipids
Dendritic Cells
Cytokines
Antigens
Galactosylceramides
Anti-Inflammatory Agents

ASJC Scopus subject areas

  • Immunology and Allergy
  • Infectious Diseases
  • Immunology

Cite this

A single subset of dendritic cells controls the cytokine bias of natural killer T cell responses to diverse glycolipid antigens. / Arora, Pooja; Baena, Andres; Yu, KarlO A.; Saini, Neeraj K.; Sharma, Shalu; Goldberg, Michael F.; Kunnath-Velayudhan, Shajo; Carreño, Leandro J.; Venkataswamy, Manjunatha M.; Kim, John; Lazar-Molnar, Eszter; Lauvau, Gregoire; Chang, Young tae; Liu, Zheng; Bittman, Robert; Al-Shamkhani, Aymen; Cox, Liam R.; Jervis, Peter J.; Veerapen, Natacha; Besra, Gurdyal S.; Porcelli, Steven A.

In: Immunity, Vol. 40, No. 1, 16.01.2014, p. 105-116.

Research output: Contribution to journalArticle

Arora, P, Baena, A, Yu, KA, Saini, NK, Sharma, S, Goldberg, MF, Kunnath-Velayudhan, S, Carreño, LJ, Venkataswamy, MM, Kim, J, Lazar-Molnar, E, Lauvau, G, Chang, YT, Liu, Z, Bittman, R, Al-Shamkhani, A, Cox, LR, Jervis, PJ, Veerapen, N, Besra, GS & Porcelli, SA 2014, 'A single subset of dendritic cells controls the cytokine bias of natural killer T cell responses to diverse glycolipid antigens', Immunity, vol. 40, no. 1, pp. 105-116. https://doi.org/10.1016/j.immuni.2013.12.004
Arora, Pooja ; Baena, Andres ; Yu, KarlO A. ; Saini, Neeraj K. ; Sharma, Shalu ; Goldberg, Michael F. ; Kunnath-Velayudhan, Shajo ; Carreño, Leandro J. ; Venkataswamy, Manjunatha M. ; Kim, John ; Lazar-Molnar, Eszter ; Lauvau, Gregoire ; Chang, Young tae ; Liu, Zheng ; Bittman, Robert ; Al-Shamkhani, Aymen ; Cox, Liam R. ; Jervis, Peter J. ; Veerapen, Natacha ; Besra, Gurdyal S. ; Porcelli, Steven A. / A single subset of dendritic cells controls the cytokine bias of natural killer T cell responses to diverse glycolipid antigens. In: Immunity. 2014 ; Vol. 40, No. 1. pp. 105-116.
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