A single point mutation in E2 enhances hepatitis C virus infectivity and alters lipoprotein association of viral particles

Wanyin Tao, Chunliang Xu, Qiang Ding, Rui Li, Yu Xiang, Josan Chung, Jin Zhong

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

Hepatitis C virus (HCV) infection is a major worldwide health problem. Our previous results showed that HCV evolved to gain the enhanced infectivity and altered buoyant density distribution during persistent infections in vitro. Here we showed that a point mutation I414T in HCV E2 was mainly responsible for these phenotypic changes. While the I414T mutation had no significant effect on HCV RNA replication and viral entry, it enhanced the production of infectious viral particles and decreased the dependency of viral entry on the levels of HCV receptors. Furthermore, we showed that the I414T mutation reduced the association of viral particles with low-density lipoprotein or very low-density lipoproteins during the virus secretion process, and the infection of the delipidated virus was more sensitive to the blockade by an anti-E2 neutralizing antibody and recombinant CD81 proteins. Our results provided more insights into understanding the roles of lipoprotein associations in HCV life cycle.

Original languageEnglish (US)
Pages (from-to)67-76
Number of pages10
JournalVirology
Volume395
Issue number1
DOIs
StatePublished - Dec 5 2009
Externally publishedYes

Fingerprint

Point Mutation
Hepacivirus
Virion
Lipoproteins
Virus Diseases
Virus Receptors
Mutation
VLDL Lipoproteins
Secretory Pathway
Viral RNA
Virus Replication
Neutralizing Antibodies
Life Cycle Stages
LDL Lipoproteins
Recombinant Proteins
Viruses
Infection

Keywords

  • E2 glycoprotein
  • Hepatitis C virus
  • Infectivity
  • Lipoprotein

ASJC Scopus subject areas

  • Virology

Cite this

A single point mutation in E2 enhances hepatitis C virus infectivity and alters lipoprotein association of viral particles. / Tao, Wanyin; Xu, Chunliang; Ding, Qiang; Li, Rui; Xiang, Yu; Chung, Josan; Zhong, Jin.

In: Virology, Vol. 395, No. 1, 05.12.2009, p. 67-76.

Research output: Contribution to journalArticle

Tao, Wanyin ; Xu, Chunliang ; Ding, Qiang ; Li, Rui ; Xiang, Yu ; Chung, Josan ; Zhong, Jin. / A single point mutation in E2 enhances hepatitis C virus infectivity and alters lipoprotein association of viral particles. In: Virology. 2009 ; Vol. 395, No. 1. pp. 67-76.
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