A Single-Molecule Surface-Based Platform to Detect the Assembly and Function of the Human RNA Polymerase II Transcription Machinery

Sang Ryul Park, Jesse Hauver, Yunxiang Zhang, Andrey Revyakin, Robert A. Coleman, Robert Tjian, Steven Chu, Alexandros Pertsinidis

Research output: Contribution to journalArticlepeer-review

Abstract

Park et al. develop optimized surface preparation procedures for single-molecule experiments. The increased biocompatibility achieved enables visualizing the assembly and function of a large, multi-component molecular machinery, the human RNA Polymerase II Transcription Pre-Initiation Complex, on a natural promoter, at the single-molecule level.

Original languageEnglish (US)
JournalStructure
DOIs
StateAccepted/In press - 2020

Keywords

  • PEG
  • Pre-Initiation Complex
  • RNA Polymerase
  • general transcription factors
  • pol II
  • polyethylene glycol
  • polymer brushes
  • single-molecule assays
  • surface passivation
  • transcription

ASJC Scopus subject areas

  • Structural Biology
  • Molecular Biology

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