A simple system for automated two-dimensional electrophoresis: applications to genetic testing.

R. K. Dhanda, W. M. Smith, C. B. Scott, C. Eng, J. Vijg

Research output: Contribution to journalArticle

12 Scopus citations

Abstract

Two-dimensional gene scanning (TDGS) is a method for mutation detection based on two-dimensional electrophoretic separation of PCR-amplified DNA fragments according to size and base pair sequence. To facilitate two-dimensional electrophoresis without manual interference, a simple three-chamber unit was designed on top of an existing vertical electrophoresis instrument. Application of this system to genetic testing is demonstrated by the performance of a complete two-dimensional mutational scanning assay of one of the hereditary nonpolyposis colon cancer syndrome susceptibility genes, hMLH1.

Original languageEnglish (US)
Pages (from-to)67-70
Number of pages4
JournalGenetic testing
Volume2
Issue number1
DOIs
Publication statusPublished - 1998
Externally publishedYes

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ASJC Scopus subject areas

  • Genetics(clinical)

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