A simple system for automated two-dimensional electrophoresis

applications to genetic testing.

R. K. Dhanda, W. M. Smith, C. B. Scott, C. Eng, Jan Vijg

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Two-dimensional gene scanning (TDGS) is a method for mutation detection based on two-dimensional electrophoretic separation of PCR-amplified DNA fragments according to size and base pair sequence. To facilitate two-dimensional electrophoresis without manual interference, a simple three-chamber unit was designed on top of an existing vertical electrophoresis instrument. Application of this system to genetic testing is demonstrated by the performance of a complete two-dimensional mutational scanning assay of one of the hereditary nonpolyposis colon cancer syndrome susceptibility genes, hMLH1.

Original languageEnglish (US)
Pages (from-to)67-70
Number of pages4
JournalGenetic Testing
Volume2
Issue number1
StatePublished - 1998
Externally publishedYes

Fingerprint

Genetic Testing
Electrophoresis
Hereditary Nonpolyposis Colorectal Neoplasms
Base Pairing
Genes
Polymerase Chain Reaction
Mutation
DNA

ASJC Scopus subject areas

  • Genetics(clinical)

Cite this

A simple system for automated two-dimensional electrophoresis : applications to genetic testing. / Dhanda, R. K.; Smith, W. M.; Scott, C. B.; Eng, C.; Vijg, Jan.

In: Genetic Testing, Vol. 2, No. 1, 1998, p. 67-70.

Research output: Contribution to journalArticle

Dhanda, R. K. ; Smith, W. M. ; Scott, C. B. ; Eng, C. ; Vijg, Jan. / A simple system for automated two-dimensional electrophoresis : applications to genetic testing. In: Genetic Testing. 1998 ; Vol. 2, No. 1. pp. 67-70.
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