A simple system for automated two-dimensional electrophoresis: applications to genetic testing.

R. K. Dhanda; W. M. Smith; C. B. Scott; C. Eng; J. Vijg

doi:10.1089/gte.1998.2.67

A simple system for automated two-dimensional electrophoresis: applications to genetic testing.

R. K. Dhanda, W. M. Smith, C. B. Scott, C. Eng, J. Vijg

Research output: Contribution to journal › Article › peer-review

12 Scopus citations

Abstract

Two-dimensional gene scanning (TDGS) is a method for mutation detection based on two-dimensional electrophoretic separation of PCR-amplified DNA fragments according to size and base pair sequence. To facilitate two-dimensional electrophoresis without manual interference, a simple three-chamber unit was designed on top of an existing vertical electrophoresis instrument. Application of this system to genetic testing is demonstrated by the performance of a complete two-dimensional mutational scanning assay of one of the hereditary nonpolyposis colon cancer syndrome susceptibility genes, hMLH1.

Original language	English (US)
Pages (from-to)	67-70
Number of pages	4
Journal	Genetic Testing
Volume	2
Issue number	1
DOIs	https://doi.org/10.1089/gte.1998.2.67
State	Published - 1998
Externally published	Yes

ASJC Scopus subject areas

Genetics(clinical)

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1089/gte.1998.2.67

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AU - Vijg, J.

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A simple system for automated two-dimensional electrophoresis: applications to genetic testing.

Abstract

ASJC Scopus subject areas

UN SDGs

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