A simple method for polyethylene glycol-promoted hybridization of mouse myeloma cells

Malcolm L. Gefter, David H. Margulies, Matthew D. Scharff

Research output: Contribution to journalArticle

431 Scopus citations

Abstract

A simple method is described for promoting the fusion of mouse myeloma cells in suspension with polyethylene glycol (PEG 1000). By carefully controlling the concentration of PEG and the time of exposure of the cells, it was possible to obtain hybridization frequencies several-hundred-fold higher than those obtained with Sendai virus.

Original languageEnglish (US)
Pages (from-to)231-236
Number of pages6
JournalSomatic Cell Genetics
Volume3
Issue number2
DOIs
StatePublished - Mar 1 1977

ASJC Scopus subject areas

  • Genetics

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