A Simple Light Stimulation of Caenorhabditis elegans

Kun He Lee, Michael Aschner

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Response via noxious stimulus can be an important indicator of sensory neuron function and overall health of an organism. If the stimulation is quick and simple, and the animal can be rescued afterwards, such a method not only allows for assays pertaining to changed sensory ability after various treatments, but also increases the reliability of the statistical relationships that are established. This protocol demonstrates a stimulation assay in Caenorhabditis elegans, using blue light from common laboratory equipment: the fluorescent microscope. The nematode detects blue light using a set of amphid ciliary sensory neurons, and blue light is detrimental to its overall health after a prolonged exposure. However, under brief exposure, blue light stimulation provides a rapid and easy method for quantifying sensory functions and health without harming the animal.

Original languageEnglish (US)
JournalCurrent Protocols in Toxicology
Volume67
DOIs
StatePublished - Feb 1 2016

Fingerprint

Caenorhabditis elegans
Light
Health
Sensory Receptor Cells
Neurons
Assays
Animals
Aptitude
Microscopes
Equipment and Supplies
Therapeutics

Keywords

  • blue light
  • Caenorhabditis elegans
  • stimulation
  • threshold

ASJC Scopus subject areas

  • Toxicology

Cite this

A Simple Light Stimulation of Caenorhabditis elegans. / Lee, Kun He; Aschner, Michael.

In: Current Protocols in Toxicology, Vol. 67, 01.02.2016.

Research output: Contribution to journalArticle

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