Abstract
Response via noxious stimulus can be an important indicator of sensory neuron function and overall health of an organism. If the stimulation is quick and simple, and the animal can be rescued afterwards, such a method not only allows for assays pertaining to changed sensory ability after various treatments, but also increases the reliability of the statistical relationships that are established. This protocol demonstrates a stimulation assay in Caenorhabditis elegans, using blue light from common laboratory equipment: the fluorescent microscope. The nematode detects blue light using a set of amphid ciliary sensory neurons, and blue light is detrimental to its overall health after a prolonged exposure. However, under brief exposure, blue light stimulation provides a rapid and easy method for quantifying sensory functions and health without harming the animal.
| Original language | English (US) |
|---|---|
| Journal | Current Protocols in Toxicology |
| Volume | 67 |
| DOIs | |
| State | Published - Feb 1 2016 |
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Keywords
- blue light
- Caenorhabditis elegans
- stimulation
- threshold
ASJC Scopus subject areas
- Toxicology
Cite this
A Simple Light Stimulation of Caenorhabditis elegans. / Lee, Kun He; Aschner, Michael.
In: Current Protocols in Toxicology, Vol. 67, 01.02.2016.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - A Simple Light Stimulation of Caenorhabditis elegans
AU - Lee, Kun He
AU - Aschner, Michael
PY - 2016/2/1
Y1 - 2016/2/1
N2 - Response via noxious stimulus can be an important indicator of sensory neuron function and overall health of an organism. If the stimulation is quick and simple, and the animal can be rescued afterwards, such a method not only allows for assays pertaining to changed sensory ability after various treatments, but also increases the reliability of the statistical relationships that are established. This protocol demonstrates a stimulation assay in Caenorhabditis elegans, using blue light from common laboratory equipment: the fluorescent microscope. The nematode detects blue light using a set of amphid ciliary sensory neurons, and blue light is detrimental to its overall health after a prolonged exposure. However, under brief exposure, blue light stimulation provides a rapid and easy method for quantifying sensory functions and health without harming the animal.
AB - Response via noxious stimulus can be an important indicator of sensory neuron function and overall health of an organism. If the stimulation is quick and simple, and the animal can be rescued afterwards, such a method not only allows for assays pertaining to changed sensory ability after various treatments, but also increases the reliability of the statistical relationships that are established. This protocol demonstrates a stimulation assay in Caenorhabditis elegans, using blue light from common laboratory equipment: the fluorescent microscope. The nematode detects blue light using a set of amphid ciliary sensory neurons, and blue light is detrimental to its overall health after a prolonged exposure. However, under brief exposure, blue light stimulation provides a rapid and easy method for quantifying sensory functions and health without harming the animal.
KW - blue light
KW - Caenorhabditis elegans
KW - stimulation
KW - threshold
UR - http://www.scopus.com/inward/record.url?scp=85020331151&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85020331151&partnerID=8YFLogxK
U2 - 10.1002/0471140856.tx1121s67
DO - 10.1002/0471140856.tx1121s67
M3 - Article
VL - 67
JO - Current protocols in toxicology / editorial board, Mahin D. Maines (editor-in-chief) ... [et al.]
JF - Current protocols in toxicology / editorial board, Mahin D. Maines (editor-in-chief) ... [et al.]
SN - 1934-9254
ER -