A simple and efficient procedure to improve plant regeneration from protoplasts isolated from long-term cell-suspension cultures of indica rice

K. Tang, E. Zhao, Q. Hu, J. Yao, A. Wu

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

A simple and efficient method has been developed to improve plant regeneration from protoplasts isolated from >1-yr-old cell-suspension culture of Indica rice (Oryza sativa) cv. Pusa Basmati 1. A two-step regeneration procedure, involving the transfer of calluses to shoot-regeneration medium containing 1% (w/v) agarose prior to culture on a medium containing 0.4% (w/v) agarose, was found to improve plant regeneration. High concentrations of kinetin in the regeneration medium were also found to be beneficial. The two-step regeneration procedure, combined with a high concentration of kinetin (10.0 mg 1 -1) in the medium, significantly increased plant regeneration. By this method, even though protoplasts were isolated from over 1-yr-old cell-suspension cultures, protoplast-derived plant regeneration frequency reached 16.1%, compared with

Original languageEnglish (US)
Pages (from-to)362-365
Number of pages4
JournalIn Vitro Cellular and Developmental Biology - Plant
Volume36
Issue number5
StatePublished - 2000
Externally publishedYes

Fingerprint

Protoplasts
cell suspension culture
protoplasts
Regeneration
Suspensions
Cell Culture Techniques
rice
kinetin
agarose
Kinetin
Sepharose
Oryza sativa
callus
shoots
Oryza
Bony Callus
methodology

Keywords

  • Cell suspensions
  • Membrane filter nurse culture
  • Oryza sativa
  • Two-step regeneration

ASJC Scopus subject areas

  • Plant Science

Cite this

A simple and efficient procedure to improve plant regeneration from protoplasts isolated from long-term cell-suspension cultures of indica rice. / Tang, K.; Zhao, E.; Hu, Q.; Yao, J.; Wu, A.

In: In Vitro Cellular and Developmental Biology - Plant, Vol. 36, No. 5, 2000, p. 362-365.

Research output: Contribution to journalArticle

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AU - Wu, A.

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