A semi-quantitative assay to screen for angiogenic compounds and compounds with angiogenic potential using the EA.hy926 endothelial cell line

Evelyn Aranda, Gareth I. Owen

Research output: Contribution to journalArticle

88 Citations (Scopus)

Abstract

Angiogenesis, the development of new capillary vessels, has a host of clinical manifestations. The identification of agents that increase or decrease angiogenesis is of great pharmaceutical interest. Classically, in vitro angiogenesis utilizes human umbilical vein endothelial cells (HUVEC) grown in matrigel. This valid and simple method has the drawbacks that each cell population is distinct and the constraint of obtaining primary source material. Herein we utilize the established EA.hy926 endothelial cell line as our model for in vitro angiogenesis and present a novel formula to quantify endothelial cell remodeling to identify pro- and anti-angiogenic agents. Furthermore, our technique details the procedures to identify and quantify compounds that have the capacity to generate pro- or anti-angiogenic factors when given to non-endothelial cells, which we define herein as angiogenic potential. In conclusion, we propose a novel formula that we are confident accurately reflects the degree of in vitro angiogenesis allowing the quantification of prospective angiogenic compounds.

Original languageEnglish (US)
Pages (from-to)377-389
Number of pages13
JournalBiological Research
Volume42
Issue number3
DOIs
StatePublished - 2009
Externally publishedYes

Fingerprint

Endothelial cells
angiogenesis
endothelial cells
Assays
Endothelial Cells
cell lines
Cell Line
assays
Cells
Angiogenesis Inducing Agents
Human Umbilical Vein Endothelial Cells
Pharmaceutical Preparations
Population
In Vitro Techniques
cells
drugs
methodology

Keywords

  • Angiogenesis
  • EA.hy926
  • Endothelial
  • Formula
  • HUVEC
  • In vitro assay
  • Matrigel

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

A semi-quantitative assay to screen for angiogenic compounds and compounds with angiogenic potential using the EA.hy926 endothelial cell line. / Aranda, Evelyn; Owen, Gareth I.

In: Biological Research, Vol. 42, No. 3, 2009, p. 377-389.

Research output: Contribution to journalArticle

@article{0becb4072e1c4e82b11cf7c2c69cb1f0,
title = "A semi-quantitative assay to screen for angiogenic compounds and compounds with angiogenic potential using the EA.hy926 endothelial cell line",
abstract = "Angiogenesis, the development of new capillary vessels, has a host of clinical manifestations. The identification of agents that increase or decrease angiogenesis is of great pharmaceutical interest. Classically, in vitro angiogenesis utilizes human umbilical vein endothelial cells (HUVEC) grown in matrigel. This valid and simple method has the drawbacks that each cell population is distinct and the constraint of obtaining primary source material. Herein we utilize the established EA.hy926 endothelial cell line as our model for in vitro angiogenesis and present a novel formula to quantify endothelial cell remodeling to identify pro- and anti-angiogenic agents. Furthermore, our technique details the procedures to identify and quantify compounds that have the capacity to generate pro- or anti-angiogenic factors when given to non-endothelial cells, which we define herein as angiogenic potential. In conclusion, we propose a novel formula that we are confident accurately reflects the degree of in vitro angiogenesis allowing the quantification of prospective angiogenic compounds.",
keywords = "Angiogenesis, EA.hy926, Endothelial, Formula, HUVEC, In vitro assay, Matrigel",
author = "Evelyn Aranda and Owen, {Gareth I.}",
year = "2009",
doi = "10.4067/S0716-97602009000300012",
language = "English (US)",
volume = "42",
pages = "377--389",
journal = "Biological Research",
issn = "0716-9760",
publisher = "Society of Biology of Chile",
number = "3",

}

TY - JOUR

T1 - A semi-quantitative assay to screen for angiogenic compounds and compounds with angiogenic potential using the EA.hy926 endothelial cell line

AU - Aranda, Evelyn

AU - Owen, Gareth I.

PY - 2009

Y1 - 2009

N2 - Angiogenesis, the development of new capillary vessels, has a host of clinical manifestations. The identification of agents that increase or decrease angiogenesis is of great pharmaceutical interest. Classically, in vitro angiogenesis utilizes human umbilical vein endothelial cells (HUVEC) grown in matrigel. This valid and simple method has the drawbacks that each cell population is distinct and the constraint of obtaining primary source material. Herein we utilize the established EA.hy926 endothelial cell line as our model for in vitro angiogenesis and present a novel formula to quantify endothelial cell remodeling to identify pro- and anti-angiogenic agents. Furthermore, our technique details the procedures to identify and quantify compounds that have the capacity to generate pro- or anti-angiogenic factors when given to non-endothelial cells, which we define herein as angiogenic potential. In conclusion, we propose a novel formula that we are confident accurately reflects the degree of in vitro angiogenesis allowing the quantification of prospective angiogenic compounds.

AB - Angiogenesis, the development of new capillary vessels, has a host of clinical manifestations. The identification of agents that increase or decrease angiogenesis is of great pharmaceutical interest. Classically, in vitro angiogenesis utilizes human umbilical vein endothelial cells (HUVEC) grown in matrigel. This valid and simple method has the drawbacks that each cell population is distinct and the constraint of obtaining primary source material. Herein we utilize the established EA.hy926 endothelial cell line as our model for in vitro angiogenesis and present a novel formula to quantify endothelial cell remodeling to identify pro- and anti-angiogenic agents. Furthermore, our technique details the procedures to identify and quantify compounds that have the capacity to generate pro- or anti-angiogenic factors when given to non-endothelial cells, which we define herein as angiogenic potential. In conclusion, we propose a novel formula that we are confident accurately reflects the degree of in vitro angiogenesis allowing the quantification of prospective angiogenic compounds.

KW - Angiogenesis

KW - EA.hy926

KW - Endothelial

KW - Formula

KW - HUVEC

KW - In vitro assay

KW - Matrigel

UR - http://www.scopus.com/inward/record.url?scp=73349137944&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=73349137944&partnerID=8YFLogxK

U2 - 10.4067/S0716-97602009000300012

DO - 10.4067/S0716-97602009000300012

M3 - Article

C2 - 19915746

AN - SCOPUS:73349137944

VL - 42

SP - 377

EP - 389

JO - Biological Research

JF - Biological Research

SN - 0716-9760

IS - 3

ER -