A role for NPY overexpression in the dorsomedial hypothalamus in hyperphagia and obesity of OLETF rats

Sheng Bi, Ellen E. Ladenheim, Gary J. Schwartz, Timothy H. Moran

Research output: Contribution to journalArticle

132 Citations (Scopus)

Abstract

Otsuka Long-Evans Tokushima Fatty (OLETF) rats lacking CCK-A receptors are hyperphagic, obese, and diabetic. We have previously demonstrated that these rats have a peripheral satiety deficit resulting in increased meal size. To examine the potential role of hypothalamic pathways in the hyperphagia and obesity of OLETF rats, we compared patterns of hypothalamic neuropeptide Y (NPY), proopiomelanocortin (POMC), and leptin receptor mRNA expression in ad libitum-fed Long-Evans Tokushima (LETO) and OLETF rats and food-restricted OLETF rats that were pair-fed to the intake of LETO controls. Pair feeding OLETF rats prevented their increased body weight and elevated levels of plasma insulin and leptin and normalized their elevated POMC and decreased NPY mRNA expression in the arcuate nucleus. In contrast, NPY expression was upregulated in the dorsomedial hypothalamus (DMH) in pair-fed OLETF rats. A similar DMH NPY overexpression was evident in 5-wk-old preobese OLETF rats. These findings suggest a role for DMH NPY upregulation in the etiology of OLETF hyperphagia and obesity.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Regulatory Integrative and Comparative Physiology
Volume281
Issue number1 50-1
StatePublished - 2001
Externally publishedYes

Fingerprint

Inbred OLETF Rats
Hyperphagia
Neuropeptide Y
Hypothalamus
Obesity
Pro-Opiomelanocortin
Cholecystokinin A Receptor
Neuropeptide Y Receptors
Leptin Receptors
Messenger RNA
Arcuate Nucleus of Hypothalamus
Leptin
Meals
Up-Regulation
Body Weight
Insulin
Food

Keywords

  • Cholecystokinin
  • Cholecystokinin-A receptor
  • Energy balance
  • In situ hybridization
  • Satiety

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)

Cite this

A role for NPY overexpression in the dorsomedial hypothalamus in hyperphagia and obesity of OLETF rats. / Bi, Sheng; Ladenheim, Ellen E.; Schwartz, Gary J.; Moran, Timothy H.

In: American Journal of Physiology - Regulatory Integrative and Comparative Physiology, Vol. 281, No. 1 50-1, 2001.

Research output: Contribution to journalArticle

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