A Role for Lipid Bodies in the Cross-presentation of Phagocytosed Antigens by MHC Class I in Dendritic Cells

Laurence Bougnères, Julie Helft, Sangeeta Tiwari, Pablo Vargas, Benny Hung Junn Chang, Lawrence Chan, Laura Campisi, Gregoire Lauvau, Stephanie Hugues, Pradeep Kumar, Alice O. Kamphorst, Ana Maria Lennon Dumenil, Michel Nussenzweig, John D. MacMicking, Sebastian Amigorena, Pierre Guermonprez

Research output: Contribution to journalArticle

78 Citations (Scopus)

Abstract

Dendritic cells (DCs) have the striking ability to cross-present exogenous antigens in association with major histocompatibility complex (MHC) class I to CD8+ T cells. However, the intracellular pathways underlying cross-presentation remain ill defined. Current models involve cytosolic proteolysis of antigens by the proteasome and peptide import into endoplasmic reticulum (ER) or phagosomal lumen by the transporters associated with antigen processing (TAP1 and TAP2). Here, we show that DCs expressed an ER-resident 47 kDa immune-related GTPase, Igtp (Irgm3). Igtp resides on ER and lipid body (LB) membranes where it binds the LB coat component ADFP. Inactivation of genes encoding for either Igtp or ADFP led to defects in LB formation in DCs and severely impaired cross-presentation of phagocytosed antigens to CD8+ T cells but not antigen presentation to CD4+ T cells. We thus define a new role for LB organelles in regulating cross-presentation of exogenous antigens to CD8+ T lymphocytes in DCs.

Original languageEnglish (US)
Pages (from-to)232-244
Number of pages13
JournalImmunity
Volume31
Issue number2
DOIs
StatePublished - Aug 21 2009
Externally publishedYes

Fingerprint

Cross-Priming
Major Histocompatibility Complex
Phagocytosis
Dendritic Cells
Endoplasmic Reticulum
CD8 Antigens
Antigens
Antigen Presentation
GTP Phosphohydrolases
Gene Silencing
Proteasome Endopeptidase Complex
Organelles
Proteolysis
T-Lymphocytes
Peptides
Membranes
Lipid Droplets

Keywords

  • CELLIMMUNO
  • MOLIMMUNO

ASJC Scopus subject areas

  • Immunology and Allergy
  • Infectious Diseases
  • Immunology

Cite this

Bougnères, L., Helft, J., Tiwari, S., Vargas, P., Chang, B. H. J., Chan, L., ... Guermonprez, P. (2009). A Role for Lipid Bodies in the Cross-presentation of Phagocytosed Antigens by MHC Class I in Dendritic Cells. Immunity, 31(2), 232-244. https://doi.org/10.1016/j.immuni.2009.06.022

A Role for Lipid Bodies in the Cross-presentation of Phagocytosed Antigens by MHC Class I in Dendritic Cells. / Bougnères, Laurence; Helft, Julie; Tiwari, Sangeeta; Vargas, Pablo; Chang, Benny Hung Junn; Chan, Lawrence; Campisi, Laura; Lauvau, Gregoire; Hugues, Stephanie; Kumar, Pradeep; Kamphorst, Alice O.; Dumenil, Ana Maria Lennon; Nussenzweig, Michel; MacMicking, John D.; Amigorena, Sebastian; Guermonprez, Pierre.

In: Immunity, Vol. 31, No. 2, 21.08.2009, p. 232-244.

Research output: Contribution to journalArticle

Bougnères, L, Helft, J, Tiwari, S, Vargas, P, Chang, BHJ, Chan, L, Campisi, L, Lauvau, G, Hugues, S, Kumar, P, Kamphorst, AO, Dumenil, AML, Nussenzweig, M, MacMicking, JD, Amigorena, S & Guermonprez, P 2009, 'A Role for Lipid Bodies in the Cross-presentation of Phagocytosed Antigens by MHC Class I in Dendritic Cells', Immunity, vol. 31, no. 2, pp. 232-244. https://doi.org/10.1016/j.immuni.2009.06.022
Bougnères, Laurence ; Helft, Julie ; Tiwari, Sangeeta ; Vargas, Pablo ; Chang, Benny Hung Junn ; Chan, Lawrence ; Campisi, Laura ; Lauvau, Gregoire ; Hugues, Stephanie ; Kumar, Pradeep ; Kamphorst, Alice O. ; Dumenil, Ana Maria Lennon ; Nussenzweig, Michel ; MacMicking, John D. ; Amigorena, Sebastian ; Guermonprez, Pierre. / A Role for Lipid Bodies in the Cross-presentation of Phagocytosed Antigens by MHC Class I in Dendritic Cells. In: Immunity. 2009 ; Vol. 31, No. 2. pp. 232-244.
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