A role for cyclooxygenase-2 in lipopolysaccharide-induced anorexia in rats

F. Lugarini, B. J. Hrupka, G. J. Schwartz, C. R. Plata-Salaman, W. Langhans

Research output: Contribution to journalArticle

78 Scopus citations

Abstract

Because nonselective cycloooxygenase (COX) inhibition attenuated anorexia after lipopolysaccharide (LPS) administration, we tested the ability of resveratrol (2.5, 10, and 40 mg/kg) and NS-398 (2.5, 10, and 40 mg/kg), selective inhibitors of the two COX isoforms COX-1 and -2, respectively, to attenuate LPS (100 μg/kg ip)-induced anorexia. NS-398 (10 and 40 mg/kg) administered with LPS at lights out attenuated LPS-induced anorexia, whereas resveratrol at all doses tested did not. Because prostaglandin (PG) E2 is considered the major metabolite synthesized by COX, we measured plasma and cerebrospinal fluid (CSF) PGE2 levels after LPS administration. LPS induced a time-dependent increase of PGE2 in CSF but not in plasma. NS-398 (5, 10, and 40 mg/kg) blocked the LPS-induced increase in CSF PGE2, whereas resveratrol (10 mg/kg) did not. These results support a role of COX-2 in mediating the anorectic response to peripheral LPS and point at PGE2 as a potential neuro-modulator involved in this response.

Original languageEnglish (US)
Pages (from-to)R862-R868
JournalAmerican Journal of Physiology - Regulatory Integrative and Comparative Physiology
Volume283
Issue number4 52-4
DOIs
StatePublished - Oct 2002

Keywords

  • Fever
  • Food intake
  • NS-398
  • Prostaglandin E
  • Resveratrol

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)

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