A Rho-dependent signaling pathway operating through myosin localizes β-actin mRNA in fibroblasts

Vaughan M. Latham, Edward H S Yu, Antonella N. Tullio, Robert S. Adelstein, Robert H. Singer

Research output: Contribution to journalArticle

65 Citations (Scopus)

Abstract

Background: The sorting of mRNA is a determinant of cell asymmetry. The cellular signals that direct specific RNA sequences to a particular cellular compartment are unknown. In fibroblasts, β-actin mRNA has been shown to be localized toward the leading edge, where it plays a role in cell motility and asymmetry. Results: We demonstrate that a signaling pathway initiated by extracellular receptors acting through Rho GTPase and Rho-kinase regulates this spatial aspect of gene expression in fibroblasts by localizing β-actin mRNA via actomyosin interactions. Consistent with the role of Rho as an activator of myosin, we found that inhibition of myosin ATPase, myosin light chain kinase (MLCK), and the knockout of myosin II-B in mouse embryonic fibroblasts all inhibited β-actin mRNA from localizing in response to growth factors. Conclusions: We therefore conclude that the sorting of β-actin mRNA in fibroblasts requires a Rho mediated pathway operating through a myosin II-B-dependent step and postulate that polarized actin bundles direct the mRNA to the leading edge of the cell.

Original languageEnglish (US)
Pages (from-to)1010-1016
Number of pages7
JournalCurrent Biology
Volume11
Issue number13
DOIs
StatePublished - Jul 10 2001

Fingerprint

Fibroblasts
Myosins
myosin
fibroblasts
actin
Actins
Nonmuscle Myosin Type IIB
Messenger RNA
RNA Transport
Myosin Type II
Sorting
myosin light chain kinase
Myosin-Light-Chain Kinase
Actomyosin
rho-Associated Kinases
rho GTP-Binding Proteins
guanosinetriphosphatase
cell movement
sorting
growth factors

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)

Cite this

A Rho-dependent signaling pathway operating through myosin localizes β-actin mRNA in fibroblasts. / Latham, Vaughan M.; Yu, Edward H S; Tullio, Antonella N.; Adelstein, Robert S.; Singer, Robert H.

In: Current Biology, Vol. 11, No. 13, 10.07.2001, p. 1010-1016.

Research output: Contribution to journalArticle

Latham, Vaughan M. ; Yu, Edward H S ; Tullio, Antonella N. ; Adelstein, Robert S. ; Singer, Robert H. / A Rho-dependent signaling pathway operating through myosin localizes β-actin mRNA in fibroblasts. In: Current Biology. 2001 ; Vol. 11, No. 13. pp. 1010-1016.
@article{9716366d204b44ccb10ce6cd6e9f3f69,
title = "A Rho-dependent signaling pathway operating through myosin localizes β-actin mRNA in fibroblasts",
abstract = "Background: The sorting of mRNA is a determinant of cell asymmetry. The cellular signals that direct specific RNA sequences to a particular cellular compartment are unknown. In fibroblasts, β-actin mRNA has been shown to be localized toward the leading edge, where it plays a role in cell motility and asymmetry. Results: We demonstrate that a signaling pathway initiated by extracellular receptors acting through Rho GTPase and Rho-kinase regulates this spatial aspect of gene expression in fibroblasts by localizing β-actin mRNA via actomyosin interactions. Consistent with the role of Rho as an activator of myosin, we found that inhibition of myosin ATPase, myosin light chain kinase (MLCK), and the knockout of myosin II-B in mouse embryonic fibroblasts all inhibited β-actin mRNA from localizing in response to growth factors. Conclusions: We therefore conclude that the sorting of β-actin mRNA in fibroblasts requires a Rho mediated pathway operating through a myosin II-B-dependent step and postulate that polarized actin bundles direct the mRNA to the leading edge of the cell.",
author = "Latham, {Vaughan M.} and Yu, {Edward H S} and Tullio, {Antonella N.} and Adelstein, {Robert S.} and Singer, {Robert H.}",
year = "2001",
month = "7",
day = "10",
doi = "10.1016/S0960-9822(01)00291-3",
language = "English (US)",
volume = "11",
pages = "1010--1016",
journal = "Current Biology",
issn = "0960-9822",
publisher = "Cell Press",
number = "13",

}

TY - JOUR

T1 - A Rho-dependent signaling pathway operating through myosin localizes β-actin mRNA in fibroblasts

AU - Latham, Vaughan M.

AU - Yu, Edward H S

AU - Tullio, Antonella N.

AU - Adelstein, Robert S.

AU - Singer, Robert H.

PY - 2001/7/10

Y1 - 2001/7/10

N2 - Background: The sorting of mRNA is a determinant of cell asymmetry. The cellular signals that direct specific RNA sequences to a particular cellular compartment are unknown. In fibroblasts, β-actin mRNA has been shown to be localized toward the leading edge, where it plays a role in cell motility and asymmetry. Results: We demonstrate that a signaling pathway initiated by extracellular receptors acting through Rho GTPase and Rho-kinase regulates this spatial aspect of gene expression in fibroblasts by localizing β-actin mRNA via actomyosin interactions. Consistent with the role of Rho as an activator of myosin, we found that inhibition of myosin ATPase, myosin light chain kinase (MLCK), and the knockout of myosin II-B in mouse embryonic fibroblasts all inhibited β-actin mRNA from localizing in response to growth factors. Conclusions: We therefore conclude that the sorting of β-actin mRNA in fibroblasts requires a Rho mediated pathway operating through a myosin II-B-dependent step and postulate that polarized actin bundles direct the mRNA to the leading edge of the cell.

AB - Background: The sorting of mRNA is a determinant of cell asymmetry. The cellular signals that direct specific RNA sequences to a particular cellular compartment are unknown. In fibroblasts, β-actin mRNA has been shown to be localized toward the leading edge, where it plays a role in cell motility and asymmetry. Results: We demonstrate that a signaling pathway initiated by extracellular receptors acting through Rho GTPase and Rho-kinase regulates this spatial aspect of gene expression in fibroblasts by localizing β-actin mRNA via actomyosin interactions. Consistent with the role of Rho as an activator of myosin, we found that inhibition of myosin ATPase, myosin light chain kinase (MLCK), and the knockout of myosin II-B in mouse embryonic fibroblasts all inhibited β-actin mRNA from localizing in response to growth factors. Conclusions: We therefore conclude that the sorting of β-actin mRNA in fibroblasts requires a Rho mediated pathway operating through a myosin II-B-dependent step and postulate that polarized actin bundles direct the mRNA to the leading edge of the cell.

UR - http://www.scopus.com/inward/record.url?scp=0035838320&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035838320&partnerID=8YFLogxK

U2 - 10.1016/S0960-9822(01)00291-3

DO - 10.1016/S0960-9822(01)00291-3

M3 - Article

C2 - 11470405

AN - SCOPUS:0035838320

VL - 11

SP - 1010

EP - 1016

JO - Current Biology

JF - Current Biology

SN - 0960-9822

IS - 13

ER -