Transient Raman spectroscopy has been used to study geminate recombination in photolyzed hemoglobins at cryogenic temperatures. The temperature dependence of the deoxy heme survival probability during a 10 ns photolysis event is analyzed in terms of a distribution of energy barriers. Studies employing different ligands (CO, O2, NO), and a mutant hemoglobin (hemoglobin Zurich) demonstrates that the barriers controlling rebinding at early times are both and protein specific.
ASJC Scopus subject areas
- Physics and Astronomy(all)
- Physical and Theoretical Chemistry