A resonance Raman study of octopus bathorhodopsin with deuterium labeled retinal chromophores.

Hua Deng, D. Manor, G. Weng, P. Rath, Y. Koutalos, T. Ebrey, R. Gebhard, J. Lugtenburg, M. Tsuda, Robert Callender

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The resonance Raman spectrum of octopus bathorhodopsin in the fingerprint region and in the ethylenic-Schiff base region have been obtained at 80 K using the "pump-probe" technique as have its deuterated chromophore analogues at the C7D; C8D; C8,C7D2; C10D; C11D; C11, C12D2; C14D; C15D; C14, C15D2; and N16D positions. While these data are not sufficient to make definitive band assignments, many tentative assignments can be made. Because of the close spectral similarity between the octopus bathorhodopsin spectrum and that of bovine bathorhodopsin, we conclude that the essential configuration of octopus bathorhodopsin's chromophore is all-trans like. The data suggest that the Schiff base, C = N, configuration is trans (anti). The observed conformationally sensitive fingerprint bands show pronounced isotope shifts upon chromophore deuteration. The size of the shifts differ, in certain cases, from those found for bovine bathorhodopsin. Thus, the internal mode composition of the fingerprint bands differs somewhat from bovine bathorhodopsin, suggesting a somewhat different in situ chromophore conformation. An analysis of the NH bend frequency, the Schiff base C = N stretch frequency, and its shift upon Schiff base deuteration suggests that the hydrogen bonding between the protonated Schiff base with its protein binding pocket is weaker in octopus bathorhodopsin than in bovine bathorhodopsin but stronger than that found in bacteriorhodopsin's bR568 pigment.

Original languageEnglish (US)
Pages (from-to)1001-1007
Number of pages7
JournalPhotochemistry and Photobiology
Volume54
Issue number6
StatePublished - Dec 1991
Externally publishedYes

Fingerprint

octopuses
Octopodiformes
Deuterium
Chromophores
imines
chromophores
deuterium
Schiff Bases
Dermatoglyphics
shift
configurations
pigments
isotope effect
Bacteriorhodopsins
bathorhodopsin
analogs
Raman spectra
pumps
proteins
Hydrogen Bonding

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biophysics

Cite this

A resonance Raman study of octopus bathorhodopsin with deuterium labeled retinal chromophores. / Deng, Hua; Manor, D.; Weng, G.; Rath, P.; Koutalos, Y.; Ebrey, T.; Gebhard, R.; Lugtenburg, J.; Tsuda, M.; Callender, Robert.

In: Photochemistry and Photobiology, Vol. 54, No. 6, 12.1991, p. 1001-1007.

Research output: Contribution to journalArticle

Deng, H, Manor, D, Weng, G, Rath, P, Koutalos, Y, Ebrey, T, Gebhard, R, Lugtenburg, J, Tsuda, M & Callender, R 1991, 'A resonance Raman study of octopus bathorhodopsin with deuterium labeled retinal chromophores.', Photochemistry and Photobiology, vol. 54, no. 6, pp. 1001-1007.
Deng, Hua ; Manor, D. ; Weng, G. ; Rath, P. ; Koutalos, Y. ; Ebrey, T. ; Gebhard, R. ; Lugtenburg, J. ; Tsuda, M. ; Callender, Robert. / A resonance Raman study of octopus bathorhodopsin with deuterium labeled retinal chromophores. In: Photochemistry and Photobiology. 1991 ; Vol. 54, No. 6. pp. 1001-1007.
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