A requirement for ARF6 in Fcγ receptor-mediated phagocytosis in macrophages

Qing Zhang, Dianne Cox, Ching Chun Tseng, Julie G. Donaldson, Steven Greenberg

Research output: Contribution to journalArticle

169 Scopus citations

Abstract

Phagocytosis requires extension of F-actin-rich pseudopods and is accompanied by membrane fusion events. Members of the ARF family of GTPases are essential for many aspects of membrane trafficking. To test a role for this family of proteins in Fcγ receptor-mediated phagocytosis, we utilized the fungal metabolite brefeldin A (BFA). The addition of 100 μM BFA to a subclone of RAW 264.7 macrophages disrupted the appearance and function of the Golgi apparatus as indicated by altered immunofluorescent distribution of β-COP and reduced efflux of BODIPY C5-ceramide, a phospholipid that normally accumulates in the Golgi apparatus. In contrast, BFA had no effect on phagocytosis of IgG-coated erythrocytes. These results suggested that activation of BFA-sensitive ARFs is not required for phagocytosis. ARF6 is unique among members of the ARF family in that its membrane association is unaffected by BFA. Expression of ARF6 mutants defective in either GTP hydrolysis (Q67L) or binding (T27N) inhibited phagocytosis of IgG-coated erythrocytes and attenuated the focal accumulation of F-actin beneath the test particles. These results indicate a requirement for ARF6 in Fcγ receptor-mediated phagocytosis and suggest that ARF6 is an important mediator of cytoskeletal alterations after Fcγ receptor activation.

Original languageEnglish (US)
Pages (from-to)19977-19981
Number of pages5
JournalJournal of Biological Chemistry
Volume273
Issue number32
DOIs
StatePublished - Aug 7 1998

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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