A reliable method for assessing topographical arrangement of proteins in the chromaffin granule membrane

Sam Zaremba; Ruth Hogue-Angeletti

doi:10.1007/BF00964769

A reliable method for assessing topographical arrangement of proteins in the chromaffin granule membrane

Sam Zaremba, Ruth Hogue-Angeletti

Developmental & Molecular Biology

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3 Scopus citations

Abstract

An efficient and reliable method for preparing chromaffin granules and membranes for topographical analysis has been designed to meet the following criteria. Only labeling of truly intact organelles is analyzed for cytoplasmic surface probes. No. resealing of open membranes occurs under specified reaction conditions. Saturation of available sites must be demonstrated. Problems in meeting these conditions are discussed, in particular the interference of granule lysate with labeling reactions.

Original language	English (US)
Pages (from-to)	19-32
Number of pages	14
Journal	Neurochemical Research
Volume	10
Issue number	1
DOIs	https://doi.org/10.1007/BF00964769
State	Published - Jan 1 1985

ASJC Scopus subject areas

Biochemistry
Cellular and Molecular Neuroscience

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10.1007/BF00964769

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title = "A reliable method for assessing topographical arrangement of proteins in the chromaffin granule membrane",

abstract = "An efficient and reliable method for preparing chromaffin granules and membranes for topographical analysis has been designed to meet the following criteria. Only labeling of truly intact organelles is analyzed for cytoplasmic surface probes. No. resealing of open membranes occurs under specified reaction conditions. Saturation of available sites must be demonstrated. Problems in meeting these conditions are discussed, in particular the interference of granule lysate with labeling reactions.",

author = "Sam Zaremba and Ruth Hogue-Angeletti",

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N2 - An efficient and reliable method for preparing chromaffin granules and membranes for topographical analysis has been designed to meet the following criteria. Only labeling of truly intact organelles is analyzed for cytoplasmic surface probes. No. resealing of open membranes occurs under specified reaction conditions. Saturation of available sites must be demonstrated. Problems in meeting these conditions are discussed, in particular the interference of granule lysate with labeling reactions.

AB - An efficient and reliable method for preparing chromaffin granules and membranes for topographical analysis has been designed to meet the following criteria. Only labeling of truly intact organelles is analyzed for cytoplasmic surface probes. No. resealing of open membranes occurs under specified reaction conditions. Saturation of available sites must be demonstrated. Problems in meeting these conditions are discussed, in particular the interference of granule lysate with labeling reactions.

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A reliable method for assessing topographical arrangement of proteins in the chromaffin granule membrane

Abstract

ASJC Scopus subject areas

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