A reliable method for assessing topographical arrangement of proteins in the chromaffin granule membrane

Sam Zaremba, Ruth Hogue-Angeletti

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

An efficient and reliable method for preparing chromaffin granules and membranes for topographical analysis has been designed to meet the following criteria. Only labeling of truly intact organelles is analyzed for cytoplasmic surface probes. No. resealing of open membranes occurs under specified reaction conditions. Saturation of available sites must be demonstrated. Problems in meeting these conditions are discussed, in particular the interference of granule lysate with labeling reactions.

Original languageEnglish (US)
Pages (from-to)19-32
Number of pages14
JournalNeurochemical Research
Volume10
Issue number1
DOIs
StatePublished - Jan 1 1985

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

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