A Regulatory Light Chain of Ciliary Outer Arm Dynein in Tetrahymena thermophila

Søren Tvorup Christensen, Charles Guerra, Yuuko Wada, Tyson Valentin, Ruth Hogue Angeletti, Peter Satir, Toshikazu Hamasaki

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Ciliary beat frequency is primarily regulated by outer arm dyneins (22 S dynein). Chilcote and Johnson (Chilcote, T. J., and Johnson, K. A. (1990) J. Biol. Chem. 256, 17257-17266) previously studied isolated Tetrahymena 22 S dynein, identifying a protein p34, which showed cAMP-dependent phosphorylation. Here, we characterize the molecular biochemistry of p34 further, demonstrating that it is the functional ortholog of the 22 S dynein regulatory light chain, p29, in Paramecium. p34, thiophosphorylated in isolated axonemes in the presence of cAMP, co-purified with 22 S dynein and not with inner arm dynein (14 S dynein). Isolated 22 S dynein containing phosphorylated p34 showed ∼70% increase in in vitro microtubule translocation velocity compared with its unphosphorylated counterpart. Extracted p34 rebound to isolated 22 S dynein from either Tetrahymena or Paramecium but not to 14 S dynein from either ciliate. Binding of radiolabeled p34 to 22 S dynein was competitive with p29. Phosphorylated p34 was not present in axonemes isolated from a mutant lacking outer arms. Two-dimensional gel electrophoresis followed by phosphorimaging revealed at least five phosphorylated p34-related spots, consistent with multiple phosphorylation sites in p34 or perhaps multiple isoforms of p34. These new features suggest that a class of outer arm dynein light chains including p34 regulates microtubule sliding velocity and consequently ciliary beat frequency through phosphorylation.

Original languageEnglish (US)
Pages (from-to)20048-20054
Number of pages7
JournalJournal of Biological Chemistry
Volume276
Issue number23
DOIs
StatePublished - Jun 8 2001

Fingerprint

Tetrahymena thermophila
Dyneins
Light
Phosphorylation
Paramecium
Axoneme
Tetrahymena
Microtubules
Biochemistry
Electrophoresis, Gel, Two-Dimensional
Electrophoresis

ASJC Scopus subject areas

  • Biochemistry

Cite this

Christensen, S. T., Guerra, C., Wada, Y., Valentin, T., Angeletti, R. H., Satir, P., & Hamasaki, T. (2001). A Regulatory Light Chain of Ciliary Outer Arm Dynein in Tetrahymena thermophila. Journal of Biological Chemistry, 276(23), 20048-20054. https://doi.org/10.1074/jbc.M008412200

A Regulatory Light Chain of Ciliary Outer Arm Dynein in Tetrahymena thermophila. / Christensen, Søren Tvorup; Guerra, Charles; Wada, Yuuko; Valentin, Tyson; Angeletti, Ruth Hogue; Satir, Peter; Hamasaki, Toshikazu.

In: Journal of Biological Chemistry, Vol. 276, No. 23, 08.06.2001, p. 20048-20054.

Research output: Contribution to journalArticle

Christensen, ST, Guerra, C, Wada, Y, Valentin, T, Angeletti, RH, Satir, P & Hamasaki, T 2001, 'A Regulatory Light Chain of Ciliary Outer Arm Dynein in Tetrahymena thermophila', Journal of Biological Chemistry, vol. 276, no. 23, pp. 20048-20054. https://doi.org/10.1074/jbc.M008412200
Christensen ST, Guerra C, Wada Y, Valentin T, Angeletti RH, Satir P et al. A Regulatory Light Chain of Ciliary Outer Arm Dynein in Tetrahymena thermophila. Journal of Biological Chemistry. 2001 Jun 8;276(23):20048-20054. https://doi.org/10.1074/jbc.M008412200
Christensen, Søren Tvorup ; Guerra, Charles ; Wada, Yuuko ; Valentin, Tyson ; Angeletti, Ruth Hogue ; Satir, Peter ; Hamasaki, Toshikazu. / A Regulatory Light Chain of Ciliary Outer Arm Dynein in Tetrahymena thermophila. In: Journal of Biological Chemistry. 2001 ; Vol. 276, No. 23. pp. 20048-20054.
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